Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers. Export

@article{citeulike:550496, abstract = {A large number of tiny noncoding RNAs have been cloned and named microRNAs (miRs). Recently, we have reported that miR-15a and miR-16a, located at 13q14, are frequently deleted and/or down-regulated in patients with B cell chronic lymphocytic leukemia, a disorder characterized by increased survival. To further investigate the possible involvement of miRs in human cancers on a genome-wide basis, we have mapped 186 miRs and compared their location to the location of previous reported nonrandom genetic alterations. Here, we show that miR genes are frequently located at fragile sites, as well as in minimal regions of loss of heterozygosity, minimal regions of amplification (minimal amplicons), or common breakpoint regions. Overall, 98 of 186 (52.5\%) of miR genes are in cancer-associated genomic regions or in fragile sites. Moreover, by Northern blotting, we have shown that several miRs located in deleted regions have low levels of expression in cancer samples. These data provide a catalog of miR genes that may have roles in cancer and argue that the full complement of miRs in a genome may be extensively involved in cancers.}, address = {Department of Microbiology and Immunology, Division of Clinical Pharmacology, Biostatistics Section, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA.}, author = {Calin, George Adrian A. and Sevignani, Cinzia and Dumitru, Calin Dan D. and Hyslop, Terry and Noch, Evan and Yendamuri, Sai and Shimizu, Masayoshi and Rattan, Sashi and Bullrich, Florencia and Negrini, Massimo and Croce, Carlo M.}, citeulike-article-id = {550496}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0307323101}, citeulike-linkout-1 = {http://www.pnas.org/cgi/content/abstract/101/9/2999}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/14973191}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=14973191}, day = {2}, doi = {10.1073/pnas.0307323101}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {cancer, mirna, mirna\_targets}, month = {March}, number = {9}, pages = {2999--3004}, posted-at = {2009-01-10 17:16:28}, priority = {2}, title = {Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers.}, url = {http://dx.doi.org/10.1073/pnas.0307323101}, volume = {101}, year = {2004} }

TY - JOUR ID - citeulike:550496 L3 - citeulike-article-id:550496 N2 - A large number of tiny noncoding RNAs have been cloned and named microRNAs (miRs). Recently, we have reported that miR-15a and miR-16a, located at 13q14, are frequently deleted and/or down-regulated in patients with B cell chronic lymphocytic leukemia, a disorder characterized by increased survival. To further investigate the possible involvement of miRs in human cancers on a genome-wide basis, we have mapped 186 miRs and compared their location to the location of previous reported nonrandom genetic alterations. Here, we show that miR genes are frequently located at fragile sites, as well as in minimal regions of loss of heterozygosity, minimal regions of amplification (minimal amplicons), or common breakpoint regions. Overall, 98 of 186 (52.5%) of miR genes are in cancer-associated genomic regions or in fragile sites. Moreover, by Northern blotting, we have shown that several miRs located in deleted regions have low levels of expression in cancer samples. These data provide a catalog of miR genes that may have roles in cancer and argue that the full complement of miRs in a genome may be extensively involved in cancers. IS - 9 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 0027-8424 AD - Department of Microbiology and Immunology, Division of Clinical Pharmacology, Biostatistics Section, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA. EP - 3004 TI - Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers. VL - 101 SP - 2999 KW - cancer KW - mirna KW - mirna_targets AU - Calin, George Adrian AU - Sevignani, Cinzia AU - Dumitru, Calin Dan AU - Hyslop, Terry AU - Noch, Evan AU - Yendamuri, Sai AU - Shimizu, Masayoshi AU - Rattan, Sashi AU - Bullrich, Florencia AU - Negrini, Massimo AU - Croce, Carlo PY - 2004/03/02/ UR - http://dx.doi.org/10.1073/pnas.0307323101 ER -