An oestrogen-receptor-alpha-bound human chromatin interactome. Export

@article{citeulike:6071519, abstract = {Genomes are organized into high-level three-dimensional structures, and DNA elements separated by long genomic distances can in principle interact functionally. Many transcription factors bind to regulatory DNA elements distant from gene promoters. Although distal binding sites have been shown to regulate transcription by long-range chromatin interactions at a few loci, chromatin interactions and their impact on transcription regulation have not been investigated in a genome-wide manner. Here we describe the development of a new strategy, chromatin interaction analysis by paired-end tag sequencing (ChIA-PET) for the de novo detection of global chromatin interactions, with which we have comprehensively mapped the chromatin interaction network bound by oestrogen receptor alpha (ER-alpha) in the human genome. We found that most high-confidence remote ER-alpha-binding sites are anchored at gene promoters through long-range chromatin interactions, suggesting that ER-alpha functions by extensive chromatin looping to bring genes together for coordinated transcriptional regulation. We propose that chromatin interactions constitute a primary mechanism for regulating transcription in mammalian genomes.}, author = {Fullwood, Melissa J. and Liu, Mei Hui H. and Pan, You Fu F. and Liu, Jun and Xu, Han and Mohamed, Yusoff Bin B. and Orlov, Yuriy L. and Velkov, Stoyan and Ho, Andrea and Mei, Poh Huay H. and Chew, Elaine G. and Huang, Phillips Yao Hui Y. and Welboren, Willem-Jan J. and Han, Yuyuan and Ooi, Hong Sain S. and Ariyaratne, Pramila N. and Vega, Vinsensius B. and Luo, Yanquan and Tan, Peck Yean Y. and Choy, Pei Ye Y. and Wansa, Senali Abayratna D. and Zhao, Bing and Lim, Kar Sian S. and Leow, Shi Chi C. and Yow, Jit Sin S. and Joseph, Roy and Li, Haixia and Desai, Kartiki V. and Thomsen, Jane S. and Lee, Yew Kok K. and Karuturi, R. Krishna Murthy and Herve, Thoreau and Bourque, Guillaume and Stunnenberg, Hendrik G. and Ruan, Xiaoan and Cacheux-Rataboul, Valere and Sung, Wing-Kin K. and Liu, Edison T. and Wei, Chia-Lin L. and Cheung, Edwin and Ruan, Yijun}, citeulike-article-id = {6071519}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nature08497}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nature08497}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19890323}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19890323}, day = {5}, doi = {10.1038/nature08497}, issn = {1476-4687}, journal = {Nature}, keywords = {chipseq, technology}, month = {November}, number = {7269}, pages = {58--64}, posted-at = {2009-11-06 19:48:00}, priority = {2}, publisher = {Nature Publishing Group}, title = {An oestrogen-receptor-alpha-bound human chromatin interactome.}, url = {http://dx.doi.org/10.1038/nature08497}, volume = {462}, year = {2009} }

TY - JOUR ID - citeulike:6071519 L3 - citeulike-article-id:6071519 N2 - Genomes are organized into high-level three-dimensional structures, and DNA elements separated by long genomic distances can in principle interact functionally. Many transcription factors bind to regulatory DNA elements distant from gene promoters. Although distal binding sites have been shown to regulate transcription by long-range chromatin interactions at a few loci, chromatin interactions and their impact on transcription regulation have not been investigated in a genome-wide manner. Here we describe the development of a new strategy, chromatin interaction analysis by paired-end tag sequencing (ChIA-PET) for the de novo detection of global chromatin interactions, with which we have comprehensively mapped the chromatin interaction network bound by oestrogen receptor alpha (ER-alpha) in the human genome. We found that most high-confidence remote ER-alpha-binding sites are anchored at gene promoters through long-range chromatin interactions, suggesting that ER-alpha functions by extensive chromatin looping to bring genes together for coordinated transcriptional regulation. We propose that chromatin interactions constitute a primary mechanism for regulating transcription in mammalian genomes. IS - 7269 JF - Nature SN - 1476-4687 EP - 64 TI - An oestrogen-receptor-alpha-bound human chromatin interactome. PB - Nature Publishing Group VL - 462 SP - 58 KW - chipseq KW - technology AU - Fullwood, Melissa AU - Liu, Mei Hui AU - Pan, You Fu AU - Liu, Jun AU - Xu, Han AU - Mohamed, Yusoff Bin AU - Orlov, Yuriy AU - Velkov, Stoyan AU - Ho, Andrea AU - Mei, Poh Huay AU - Chew, Elaine AU - Huang, Phillips Yao Hui AU - Welboren, Willem-Jan AU - Han, Yuyuan AU - Ooi, Hong Sain AU - Ariyaratne, Pramila AU - Vega, Vinsensius AU - Luo, Yanquan AU - Tan, Peck Yean AU - Choy, Pei Ye AU - Wansa, Senali Abayratna AU - Zhao, Bing AU - Lim, Kar Sian AU - Leow, Shi Chi AU - Yow, Jit Sin AU - Joseph, Roy AU - Li, Haixia AU - Desai, Kartiki AU - Thomsen, Jane AU - Lee, Yew Kok AU - Karuturi, Krishna Murthy AU - Herve, Thoreau AU - Bourque, Guillaume AU - Stunnenberg, Hendrik AU - Ruan, Xiaoan AU - Cacheux-Rataboul, Valere AU - Sung, Wing-Kin AU - Liu, Edison AU - Wei, Chia-Lin AU - Cheung, Edwin AU - Ruan, Yijun PY - 2009/11/05/ UR - http://dx.doi.org/10.1038/nature08497 ER -