Bidirectional silencing of RNA polymerase I transcription by a strand switch region in Trypanosoma brucei Export

@article{citeulike:5650288, abstract = {The procyclin genes in Trypanosoma brucei are transcribed by RNA polymerase I as part of 5-10 kb long polycistronic transcription units on chromosomes VI and X. Each procyclin locus begins with two procyclin genes followed by at least one procyclin-associated gene (PAG). In procyclic (insect midgut) form trypanosomes, PAG mRNA levels are about 100-fold lower than those of procyclins. We show that deletion of PAG1, PAG2 or PAG3 results in increased mRNA levels from downstream genes in the same transcription unit. Nascent RNA analysis revealed that most of the effects are due to increased transcription elongation in the knockouts. Furthermore, transient and stable transfections showed that sequence elements on both strands of PAG1 can inhibit Pol I transcription. Finally, by database mining we identified 30 additional PAG-related sequences that are located almost exclusively at strand switch regions and/or at sites where a change of RNA polymerase type is likely to occur. 10.1093/nar/gkp513}, author = {Haenni, Simon and Studer, Erwin and Burkard, Gabriela S. and Roditi, Isabel}, citeulike-article-id = {5650288}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/nar/gkp513}, citeulike-linkout-1 = {http://nar.oxfordjournals.org/cgi/content/abstract/37/15/5007}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19531741}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19531741}, day = {1}, doi = {10.1093/nar/gkp513}, journal = {Nucl. Acids Res.}, keywords = {brucei, silencing, strand, switch, transcription, trypanosoma}, month = {August}, number = {15}, pages = {5007--5018}, posted-at = {2009-08-26 13:05:10}, priority = {3}, title = {Bidirectional silencing of RNA polymerase I transcription by a strand switch region in Trypanosoma brucei}, url = {http://dx.doi.org/10.1093/nar/gkp513}, volume = {37}, year = {2009} }

TY - JOUR ID - citeulike:5650288 L3 - citeulike-article-id:5650288 N2 - The procyclin genes in Trypanosoma brucei are transcribed by RNA polymerase I as part of 5-10 kb long polycistronic transcription units on chromosomes VI and X. Each procyclin locus begins with two procyclin genes followed by at least one procyclin-associated gene (PAG). In procyclic (insect midgut) form trypanosomes, PAG mRNA levels are about 100-fold lower than those of procyclins. We show that deletion of PAG1, PAG2 or PAG3 results in increased mRNA levels from downstream genes in the same transcription unit. Nascent RNA analysis revealed that most of the effects are due to increased transcription elongation in the knockouts. Furthermore, transient and stable transfections showed that sequence elements on both strands of PAG1 can inhibit Pol I transcription. Finally, by database mining we identified 30 additional PAG-related sequences that are located almost exclusively at strand switch regions and/or at sites where a change of RNA polymerase type is likely to occur. 10.1093/nar/gkp513 IS - 15 JF - Nucl. Acids Res. EP - 5018 TI - Bidirectional silencing of RNA polymerase I transcription by a strand switch region in Trypanosoma brucei VL - 37 SP - 5007 KW - brucei KW - silencing KW - strand KW - switch KW - transcription KW - trypanosoma AU - Haenni, Simon AU - Studer, Erwin AU - Burkard, Gabriela AU - Roditi, Isabel PY - 2009/08/01/ UR - http://dx.doi.org/10.1093/nar/gkp513 ER -