The fluxes through glycolytic enzymes in Saccharomyces cerevisiae are predominantly regulated at posttranscriptional levels Export

@article{citeulike:1891855, abstract = {Metabolic fluxes may be regulated "hierarchically," e.g., by changes of gene expression that adjust enzyme capacities (Vmax) and/or "metabolically" by interactions of enzymes with substrates, products, or allosteric effectors. In the present study, a method is developed to dissect the hierarchical regulation into contributions by transcription, translation, protein degradation, and posttranslational modification. The method was applied to the regulation of fluxes through individual glycolytic enzymes when the yeast Saccharomyces cerevisiae was confronted with the absence of oxygen and the presence of benzoic acid depleting its ATP. Metabolic regulation largely contributed to the {approx}10-fold change in flux through the glycolytic enzymes. This contribution varied from 50 to 80\%, depending on the glycolytic step and the cultivation condition tested. Within the 5020\% hierarchical regulation of fluxes, transcription played a minor role, whereas regulation of protein synthesis or degradation was the most important. These also contributed to 75100\% of the regulation of protein levels. 10.1073/pnas.0707476104}, author = {Daran-Lapujade, Pascale and Rossell, Sergio and van Gulik, Walter M. and Luttik, Marijke A. and de Groot, Marco J. and Slijper, Monique and Heck, Albert J. and Daran, Jean-Marc and de Winde, Johannes H. and Westerhoff, Hans V. and Pronk, Jack T. and Bakker, Barbara M.}, citeulike-article-id = {1891855}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0707476104}, citeulike-linkout-1 = {http://www.pnas.org/cgi/content/abstract/104/40/15753}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17898166}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17898166}, day = {2}, doi = {10.1073/pnas.0707476104}, journal = {Proceedings of the National Academy of Sciences}, keywords = {flux\_regulation}, month = {October}, number = {40}, pages = {15753--15758}, posted-at = {2007-11-10 01:36:13}, priority = {0}, title = {The fluxes through glycolytic enzymes in Saccharomyces cerevisiae are predominantly regulated at posttranscriptional levels}, url = {http://dx.doi.org/10.1073/pnas.0707476104}, volume = {104}, year = {2007} }

TY - JOUR ID - citeulike:1891855 L3 - citeulike-article-id:1891855 N2 - Metabolic fluxes may be regulated "hierarchically," e.g., by changes of gene expression that adjust enzyme capacities (Vmax) and/or "metabolically" by interactions of enzymes with substrates, products, or allosteric effectors. In the present study, a method is developed to dissect the hierarchical regulation into contributions by transcription, translation, protein degradation, and posttranslational modification. The method was applied to the regulation of fluxes through individual glycolytic enzymes when the yeast Saccharomyces cerevisiae was confronted with the absence of oxygen and the presence of benzoic acid depleting its ATP. Metabolic regulation largely contributed to the {approx}10-fold change in flux through the glycolytic enzymes. This contribution varied from 50 to 80%, depending on the glycolytic step and the cultivation condition tested. Within the 5020% hierarchical regulation of fluxes, transcription played a minor role, whereas regulation of protein synthesis or degradation was the most important. These also contributed to 75100% of the regulation of protein levels. 10.1073/pnas.0707476104 IS - 40 JF - Proceedings of the National Academy of Sciences EP - 15758 TI - The fluxes through glycolytic enzymes in Saccharomyces cerevisiae are predominantly regulated at posttranscriptional levels VL - 104 SP - 15753 KW - flux_regulation AU - Daran-Lapujade, Pascale AU - Rossell, Sergio AU - van Gulik, Walter AU - Luttik, Marijke AU - de Groot, Marco AU - Slijper, Monique AU - Heck, Albert AU - Daran, Jean-Marc AU - de Winde, Johannes AU - Westerhoff, Hans AU - Pronk, Jack AU - Bakker, Barbara PY - 2007/10/02/ UR - http://dx.doi.org/10.1073/pnas.0707476104 ER -