The Escherichia coli MG1655 in silico metabolic genotype: Its definition, characteristics, and capabilities Export

@article{citeulike:581018, abstract = {The MG1655 genome has been completely sequenced. The annotated sequence, biochemical information, and other information were used to reconstruct the metabolic map. The stoichiometric coefficients for each metabolic enzyme in the metabolic map were assembled to construct a genome-specific stoichiometric matrix. The stoichiometric matrix was used to define the system's characteristics and the capabilities of metabolism. The effects of gene deletions in the central metabolic pathways on the ability of the metabolic network to support growth were assessed, and the predictions were compared with experimental observations. It was shown that based on stoichiometric and capacity constraints the analysis was able to qualitatively predict the growth potential of mutant strains in 86\% of the cases examined. Herein, it is demonstrated that the synthesis of metabolic genotypes based on genomic, biochemical, and strain-specific information is possible, and that systems analysis methods are available to analyze and interpret the metabolic phenotype.}, author = {Edwards, J. S. and Palsson, B. O.}, citeulike-article-id = {581018}, citeulike-linkout-0 = {http://www.pnas.org/content/97/10/5528.abstract}, citeulike-linkout-1 = {http://www.pnas.org/content/97/10/5528.full.pdf}, citeulike-linkout-2 = {http://www.pnas.org/cgi/content/abstract/97/10/5528}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/10805808}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=10805808}, day = {9}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {ecoli\_ppp, fba}, month = {May}, number = {10}, pages = {5528--5533}, posted-at = {2008-04-18 00:37:11}, priority = {0}, title = {The Escherichia coli MG1655 in silico metabolic genotype: Its definition, characteristics, and capabilities}, url = {http://www.pnas.org/content/97/10/5528.abstract}, volume = {97}, year = {2000} }

TY - JOUR ID - citeulike:581018 L3 - citeulike-article-id:581018 N2 - The MG1655 genome has been completely sequenced. The annotated sequence, biochemical information, and other information were used to reconstruct the metabolic map. The stoichiometric coefficients for each metabolic enzyme in the metabolic map were assembled to construct a genome-specific stoichiometric matrix. The stoichiometric matrix was used to define the system's characteristics and the capabilities of metabolism. The effects of gene deletions in the central metabolic pathways on the ability of the metabolic network to support growth were assessed, and the predictions were compared with experimental observations. It was shown that based on stoichiometric and capacity constraints the analysis was able to qualitatively predict the growth potential of mutant strains in 86% of the cases examined. Herein, it is demonstrated that the synthesis of metabolic genotypes based on genomic, biochemical, and strain-specific information is possible, and that systems analysis methods are available to analyze and interpret the metabolic phenotype. IS - 10 JF - Proceedings of the National Academy of Sciences of the United States of America EP - 5533 TI - The Escherichia coli MG1655 in silico metabolic genotype: Its definition, characteristics, and capabilities VL - 97 SP - 5528 KW - ecoli_ppp KW - fba AU - Edwards, JS AU - Palsson, BO PY - 2000/05/09/ UR - http://www.pnas.org/content/97/10/5528.abstract ER -