Inhibition of kinesin synthesis and rapid anterograde axonal transport in vivo by an antisense oligonucleotide. Export

@article{citeulike:6097312, abstract = {Synthetic antisense oligonucleotides have been used to inhibit specific protein synthesis in vivo. Antisense oligonucleotides directed to kinesin heavy chain were injected into the vitreous of anesthetized rabbits in order to assess the effects on transport in the retinal ganglion cells whose axons form the optic nerve. The antisense oligonucleotide specifically inhibited retinal kinesin synthesis by 82 +/- 7\% (n = 4). The rapid axonal transport of the membrane proteins into the optic nerve was concomitantly inhibited by 70 +/- 10\% (n = 4). These results provide direct evidence for the specific role of kinesin in rapid anterograde transport in vivo and indicate the utility of antisense oligonucleotides to explore neuronal dynamics in a specific neuronal cell type in a living animal.}, author = {Amaratunga, A. and Morin, P. J. and Kosik, K. S. and Fine, R. E.}, citeulike-article-id = {6097312}, citeulike-linkout-0 = {http://www.jbc.org/content/268/23/17427.abstract}, citeulike-linkout-1 = {http://www.jbc.org/content/268/23/17427.full.pdf}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/7688725}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=7688725}, day = {15}, journal = {Journal of Biological Chemistry}, keywords = {axon, axons, kinesin, microtuble, motor}, month = {August}, number = {23}, pages = {17427--17430}, posted-at = {2009-11-11 10:34:39}, priority = {2}, title = {Inhibition of kinesin synthesis and rapid anterograde axonal transport in vivo by an antisense oligonucleotide.}, url = {http://www.jbc.org/content/268/23/17427.abstract}, volume = {268}, year = {1993} }

TY - JOUR ID - citeulike:6097312 L3 - citeulike-article-id:6097312 N2 - Synthetic antisense oligonucleotides have been used to inhibit specific protein synthesis in vivo. Antisense oligonucleotides directed to kinesin heavy chain were injected into the vitreous of anesthetized rabbits in order to assess the effects on transport in the retinal ganglion cells whose axons form the optic nerve. The antisense oligonucleotide specifically inhibited retinal kinesin synthesis by 82 +/- 7% (n = 4). The rapid axonal transport of the membrane proteins into the optic nerve was concomitantly inhibited by 70 +/- 10% (n = 4). These results provide direct evidence for the specific role of kinesin in rapid anterograde transport in vivo and indicate the utility of antisense oligonucleotides to explore neuronal dynamics in a specific neuronal cell type in a living animal. IS - 23 JF - Journal of Biological Chemistry EP - 17430 TI - Inhibition of kinesin synthesis and rapid anterograde axonal transport in vivo by an antisense oligonucleotide. VL - 268 SP - 17427 KW - axon KW - axons KW - kinesin KW - microtuble KW - motor AU - Amaratunga, A AU - Morin, PJ AU - Kosik, KS AU - Fine, RE PY - 1993/08/15/ UR - http://www.jbc.org/content/268/23/17427.abstract ER -