Global mapping of c-Myc binding sites and target gene networks in human B cells. Export

@article{citeulike:1270341, abstract = {The protooncogene MYC encodes the c-Myc transcription factor that regulates cell growth, cell proliferation, cell cycle, and apoptosis. Although deregulation of MYC contributes to tumorigenesis, it is still unclear what direct Myc-induced transcriptomes promote cell transformation. Here we provide a snapshot of genome-wide, unbiased characterization of direct Myc binding targets in a model of human B lymphoid tumor using ChIP coupled with pair-end ditag sequencing analysis (ChIP-PET). Myc potentially occupies > 4,000 genomic loci with the majority near proximal promoter regions associated frequently with CpG islands. Using gene expression profiles with ChIP-PET, we identified 668 direct Myc-regulated gene targets, including 48 transcription factors, indicating that Myc is a central transcriptional hub in growth and proliferation control. This first global genomic view of Myc binding sites yields insights of transcriptional circuitries and cis regulatory modules involving Myc and provides a substantial framework for our understanding of mechanisms of Myc-induced tumorigenesis.}, address = {Department of Medicine and The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.}, author = {Zeller, Karen I. and Zhao, XiaoDong and Lee, Charlie W. and Chiu, Kuo Ping P. and Yao, Fei and Yustein, Jason T. and Ooi, Hong Sain S. and Orlov, Yuriy L. and Shahab, Atif and Yong, How Choong C. and Fu, Yutao and Weng, Zhiping and Kuznetsov, Vladimir A. and Sung, Wing-Kin K. and Ruan, Yijun and Dang, Chi V. and Wei, Chia-Lin L.}, citeulike-article-id = {1270341}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0604129103}, citeulike-linkout-1 = {http://www.pnas.org/content/103/47/17834.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/103/47/17834.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/17093053}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=17093053}, day = {21}, doi = {10.1073/pnas.0604129103}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {chip, chip\_pet, myc, network, target\_genes}, month = {November}, number = {47}, pages = {17834--17839}, posted-at = {2009-10-28 17:10:35}, priority = {2}, title = {Global mapping of c-Myc binding sites and target gene networks in human B cells.}, url = {http://dx.doi.org/10.1073/pnas.0604129103}, volume = {103}, year = {2006} }

TY - JOUR ID - citeulike:1270341 L3 - citeulike-article-id:1270341 N2 - The protooncogene MYC encodes the c-Myc transcription factor that regulates cell growth, cell proliferation, cell cycle, and apoptosis. Although deregulation of MYC contributes to tumorigenesis, it is still unclear what direct Myc-induced transcriptomes promote cell transformation. Here we provide a snapshot of genome-wide, unbiased characterization of direct Myc binding targets in a model of human B lymphoid tumor using ChIP coupled with pair-end ditag sequencing analysis (ChIP-PET). Myc potentially occupies > 4,000 genomic loci with the majority near proximal promoter regions associated frequently with CpG islands. Using gene expression profiles with ChIP-PET, we identified 668 direct Myc-regulated gene targets, including 48 transcription factors, indicating that Myc is a central transcriptional hub in growth and proliferation control. This first global genomic view of Myc binding sites yields insights of transcriptional circuitries and cis regulatory modules involving Myc and provides a substantial framework for our understanding of mechanisms of Myc-induced tumorigenesis. IS - 47 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 0027-8424 AD - Department of Medicine and The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. EP - 17839 TI - Global mapping of c-Myc binding sites and target gene networks in human B cells. VL - 103 SP - 17834 KW - chip KW - chip_pet KW - myc KW - network KW - target_genes AU - Zeller, Karen AU - Zhao, XiaoDong AU - Lee, Charlie AU - Chiu, Kuo Ping AU - Yao, Fei AU - Yustein, Jason AU - Ooi, Hong Sain AU - Orlov, Yuriy AU - Shahab, Atif AU - Yong, How Choong AU - Fu, Yutao AU - Weng, Zhiping AU - Kuznetsov, Vladimir AU - Sung, Wing-Kin AU - Ruan, Yijun AU - Dang, Chi AU - Wei, Chia-Lin PY - 2006/11/21/ UR - http://dx.doi.org/10.1073/pnas.0604129103 ER -