Human induced pluripotent stem cells free of vector and transgene sequences. Export

@article{citeulike:4491138, abstract = {Reprogramming differentiated human cells to induced pluripotent stem (iPS) cells has applications in basic biology, drug development, and transplantation. Human iPS cell derivation previously required vectors that integrate into the genome, which can create mutations and limit the utility of the cells in both research and clinical applications. We describe the derivation of human iPS cells with the use of nonintegrating episomal vectors. After removal of the episome, iPS cells completely free of vector and transgene sequences are derived that are similar to human embryonic stem (ES) cells in proliferative and developmental potential. These results demonstrate that reprogramming human somatic cells does not require genomic integration or the continued presence of exogenous reprogramming factors and removes one obstacle to the clinical application of human iPS cells.}, author = {Yu, Junying and Hu, Kejin and Smuga-Otto, Kim and Tian, Shulan and Stewart, Ron and Slukvin, Igor I. and Thomson, James A.}, citeulike-article-id = {4491138}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1172482}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/324/5928/797?sa_campaign=Email}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19325077}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19325077}, day = {8}, doi = {10.1126/science.1172482}, issn = {1095-9203}, journal = {Science (New York, N.Y.)}, keywords = {stemcell}, month = {May}, number = {5928}, pages = {797--801}, posted-at = {2009-05-27 21:33:51}, priority = {2}, title = {Human induced pluripotent stem cells free of vector and transgene sequences.}, url = {http://dx.doi.org/10.1126/science.1172482}, volume = {324}, year = {2009} }

TY - JOUR ID - citeulike:4491138 L3 - citeulike-article-id:4491138 N2 - Reprogramming differentiated human cells to induced pluripotent stem (iPS) cells has applications in basic biology, drug development, and transplantation. Human iPS cell derivation previously required vectors that integrate into the genome, which can create mutations and limit the utility of the cells in both research and clinical applications. We describe the derivation of human iPS cells with the use of nonintegrating episomal vectors. After removal of the episome, iPS cells completely free of vector and transgene sequences are derived that are similar to human embryonic stem (ES) cells in proliferative and developmental potential. These results demonstrate that reprogramming human somatic cells does not require genomic integration or the continued presence of exogenous reprogramming factors and removes one obstacle to the clinical application of human iPS cells. IS - 5928 JF - Science (New York, N.Y.) SN - 1095-9203 EP - 801 TI - Human induced pluripotent stem cells free of vector and transgene sequences. VL - 324 SP - 797 KW - stemcell AU - Yu, Junying AU - Hu, Kejin AU - Smuga-Otto, Kim AU - Tian, Shulan AU - Stewart, Ron AU - Slukvin, Igor AU - Thomson, James PY - 2009/05/08/ UR - http://dx.doi.org/10.1126/science.1172482 ER -