Coding-sequence determinants of gene expression in Escherichia coli. Export

@article{citeulike:4295691, abstract = {Synonymous mutations do not alter the encoded protein, but they can influence gene expression. To investigate how, we engineered a synthetic library of 154 genes that varied randomly at synonymous sites, but all encoded the same green fluorescent protein (GFP). When expressed in Escherichia coli, GFP protein levels varied 250-fold across the library. GFP messenger RNA (mRNA) levels, mRNA degradation patterns, and bacterial growth rates also varied, but codon bias did not correlate with gene expression. Rather, the stability of mRNA folding near the ribosomal binding site explained more than half the variation in protein levels. In our analysis, mRNA folding and associated rates of translation initiation play a predominant role in shaping expression levels of individual genes, whereas codon bias influences global translation efficiency and cellular fitness.}, author = {Kudla, Grzegorz and Murray, Andrew W. and Tollervey, David and Plotkin, Joshua B.}, citeulike-article-id = {4295691}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1170160}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/324/5924/255}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19359587}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19359587}, day = {10}, doi = {10.1126/science.1170160}, issn = {1095-9203}, journal = {Science (New York, N.Y.)}, keywords = {coding, expression, gene, sequence}, month = {April}, number = {5924}, pages = {255--258}, posted-at = {2009-04-10 02:22:29}, priority = {2}, title = {Coding-sequence determinants of gene expression in Escherichia coli.}, url = {http://dx.doi.org/10.1126/science.1170160}, volume = {324}, year = {2009} }

TY - JOUR ID - citeulike:4295691 L3 - citeulike-article-id:4295691 N2 - Synonymous mutations do not alter the encoded protein, but they can influence gene expression. To investigate how, we engineered a synthetic library of 154 genes that varied randomly at synonymous sites, but all encoded the same green fluorescent protein (GFP). When expressed in Escherichia coli, GFP protein levels varied 250-fold across the library. GFP messenger RNA (mRNA) levels, mRNA degradation patterns, and bacterial growth rates also varied, but codon bias did not correlate with gene expression. Rather, the stability of mRNA folding near the ribosomal binding site explained more than half the variation in protein levels. In our analysis, mRNA folding and associated rates of translation initiation play a predominant role in shaping expression levels of individual genes, whereas codon bias influences global translation efficiency and cellular fitness. IS - 5924 JF - Science (New York, N.Y.) SN - 1095-9203 EP - 258 TI - Coding-sequence determinants of gene expression in Escherichia coli. VL - 324 SP - 255 KW - coding KW - expression KW - gene KW - sequence AU - Kudla, Grzegorz AU - Murray, Andrew AU - Tollervey, David AU - Plotkin, Joshua PY - 2009/04/10/ UR - http://dx.doi.org/10.1126/science.1170160 ER -