Oligonucleotides derived from the packaging signal at the 5' end of the viral PB2 segment specifically inhibit influenza virus in vitro. Export

@article{citeulike:4361348, abstract = {The development of new antiviral molecules to fight the possible emergence of influenza viruses with pandemic potential is needed. In this study, phosphorothioate oligonucleotides (S-ONs) derived from the packaging signals in the 3' and 5' ends of the viral PB2 RNA were associated with liposomes and tested against influenza virus in vitro. A 15-mer S-ON derived from the 5' end of the viral PB2 RNA, complementary to the 3' end of its coding region (nucleotides 2279-2293) and designated 5-15b, proved markedly inhibitory. The antiviral activity of 5-15b was dose- and time-dependent but was independent of the cell substrate and multiplicity of infection used. Importantly, inhibition of influenza A and B viruses required S-ONs reproducing the respective packaging signals. Furthermore, 5-15b and its antisense derivative S-ON activity did not affect intracellular accumulation of viral RNA. Confocal microscopy showed that 5-15b is clearly nucleophilic. These findings indicate that the packaging signal at the 5' end of the PB2 RNA is an interesting target for the design of new anti-influenza-virus compounds.}, author = {Giannecchini, Simone and Clausi, Valeria and Nosi, Daniele and Azzi, Alberta}, citeulike-article-id = {4361348}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s00705-009-0380-2}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19370391}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19370391}, day = {16}, doi = {10.1007/s00705-009-0380-2}, issn = {1432-8798}, journal = {Archives of virology}, keywords = {drug, influenza, pb2, vaccine}, month = {April}, posted-at = {2009-04-18 08:04:05}, priority = {2}, title = {Oligonucleotides derived from the packaging signal at the 5' end of the viral PB2 segment specifically inhibit influenza virus in vitro.}, url = {http://dx.doi.org/10.1007/s00705-009-0380-2}, year = {2009} }

TY - JOUR ID - citeulike:4361348 L3 - citeulike-article-id:4361348 N2 - The development of new antiviral molecules to fight the possible emergence of influenza viruses with pandemic potential is needed. In this study, phosphorothioate oligonucleotides (S-ONs) derived from the packaging signals in the 3' and 5' ends of the viral PB2 RNA were associated with liposomes and tested against influenza virus in vitro. A 15-mer S-ON derived from the 5' end of the viral PB2 RNA, complementary to the 3' end of its coding region (nucleotides 2279-2293) and designated 5-15b, proved markedly inhibitory. The antiviral activity of 5-15b was dose- and time-dependent but was independent of the cell substrate and multiplicity of infection used. Importantly, inhibition of influenza A and B viruses required S-ONs reproducing the respective packaging signals. Furthermore, 5-15b and its antisense derivative S-ON activity did not affect intracellular accumulation of viral RNA. Confocal microscopy showed that 5-15b is clearly nucleophilic. These findings indicate that the packaging signal at the 5' end of the PB2 RNA is an interesting target for the design of new anti-influenza-virus compounds. JF - Archives of virology SN - 1432-8798 TI - Oligonucleotides derived from the packaging signal at the 5' end of the viral PB2 segment specifically inhibit influenza virus in vitro. KW - drug KW - influenza KW - pb2 KW - vaccine AU - Giannecchini, Simone AU - Clausi, Valeria AU - Nosi, Daniele AU - Azzi, Alberta PY - 2009/04/16/ UR - http://dx.doi.org/10.1007/s00705-009-0380-2 ER -