MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias. Export

@article{citeulike:550494, abstract = {Little is known about the expression levels or function of micro-RNAs (miRNAs) in normal and neoplastic cells, although it is becoming clear that miRNAs play important roles in the regulation of gene expression during development [Ambros, V. (2003) Cell 113, 673-676; McManus, M. T. (2003) Semin. Cancer Biol. 13, 253-258]. We now report the genomewide expression profiling of miRNAs in human B cell chronic lymphocytic leukemia (CLL) by using a microarray containing hundreds of human precursor and mature miRNA oligonucleotide probes. This approach allowed us to identify significant differences in miRNome expression between CLL samples and normal CD5+ B cells; data were confirmed by Northern blot analyses and real-time RT-PCR. At least two distinct clusters of CLL samples can be identified that were associated with the presence or absence of Zap-70 expression, a predictor of early disease progression. Two miRNA signatures were associated with the presence or absence of mutations in the expressed Ig variableregion genes or with deletions at 13q14, respectively. These data suggest that miRNA expression patterns have relevance to the biological and clinical behavior of this leukemia.}, address = {Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA.}, author = {Calin, G. A. and Liu, C. G. and Sevignani, C. and Ferracin, M. and Felli, N. and Dumitru, C. D. and Shimizu, M. and Cimmino, A. and Zupo, S. and Dono, M. and Dell'Aquila, M. L. and Alder, H. and Rassenti, L. and Kipps, T. J. and Bullrich, F. and Negrini, M. and Croce, C. M.}, citeulike-article-id = {550494}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0404432101}, citeulike-linkout-1 = {http://www.pnas.org/content/101/32/11755.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/101/32/11755.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/15284443}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=15284443}, day = {10}, doi = {10.1073/pnas.0404432101}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {leukemia, microrna, prognosis}, month = {August}, number = {32}, pages = {11755--11760}, posted-at = {2008-09-15 13:01:46}, priority = {2}, title = {MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias.}, url = {http://dx.doi.org/10.1073/pnas.0404432101}, volume = {101}, year = {2004} }

TY - JOUR ID - citeulike:550494 L3 - citeulike-article-id:550494 N2 - Little is known about the expression levels or function of micro-RNAs (miRNAs) in normal and neoplastic cells, although it is becoming clear that miRNAs play important roles in the regulation of gene expression during development [Ambros, V. (2003) Cell 113, 673-676; McManus, M. T. (2003) Semin. Cancer Biol. 13, 253-258]. We now report the genomewide expression profiling of miRNAs in human B cell chronic lymphocytic leukemia (CLL) by using a microarray containing hundreds of human precursor and mature miRNA oligonucleotide probes. This approach allowed us to identify significant differences in miRNome expression between CLL samples and normal CD5+ B cells; data were confirmed by Northern blot analyses and real-time RT-PCR. At least two distinct clusters of CLL samples can be identified that were associated with the presence or absence of Zap-70 expression, a predictor of early disease progression. Two miRNA signatures were associated with the presence or absence of mutations in the expressed Ig variableregion genes or with deletions at 13q14, respectively. These data suggest that miRNA expression patterns have relevance to the biological and clinical behavior of this leukemia. IS - 32 JF - Proc Natl Acad Sci U S A SN - 0027-8424 AD - Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA. EP - 11760 TI - MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias. VL - 101 SP - 11755 KW - leukemia KW - microrna KW - prognosis AU - Calin, GA AU - Liu, CG AU - Sevignani, C AU - Ferracin, M AU - Felli, N AU - Dumitru, CD AU - Shimizu, M AU - Cimmino, A AU - Zupo, S AU - Dono, M AU - Dell'Aquila, ML AU - Alder, H AU - Rassenti, L AU - Kipps, TJ AU - Bullrich, F AU - Negrini, M AU - Croce, CM PY - 2004/08/10/ UR - http://dx.doi.org/10.1073/pnas.0404432101 ER -