Heterochromatic Silencing and HP1 Localization in Drosophila Are Dependent on the RNAi Machinery Export

@article{palbhadra2004, abstract = {Genes normally resident in euchromatic domains are silenced when packaged into heterochromatin, as exemplified in Drosophila melanogaster by position effect variegation (PEV). Loss-of-function mutations resulting in suppression of PEV have identified critical components of heterochromatin, including proteins HP1, HP2, and histone H3 lysine 9 methyltransferase. Here, we demonstrate that this silencing is dependent on the RNA interference machinery, using tandem mini-white arrays and white transgenes in heterochromatin to show loss of silencing as a result of mutations in piwi, aubergine, or spindle-E (homeless), which encode RNAi components. These mutations result in reduction of H3 Lys9 methylation and delocalization of HP1 and HP2, most dramatically in spindle-E mutants. 10.1126/science.1092653}, address = {Division of Biological Sciences, 117 Tucker Hall, University of Missouri, Columbia, MO 65211, USA.}, author = {Pal-Bhadra, Manika and Leibovitch, Boris A. and Gandhi, Sumit G. and Rao, Madhusudana and Bhadra, Utpal and Birchler, James A. and Elgin, Sarah C.}, citeulike-article-id = {140166}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1092653}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/303/5658/669}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/14752161}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=14752161}, doi = {10.1126/science.1092653}, issn = {1095-9203}, journal = {Science}, keywords = {chromatin, drosophila, hp1, rnai}, month = {January}, number = {5658}, pages = {669--672}, posted-at = {2009-07-02 12:49:00}, priority = {3}, title = {Heterochromatic Silencing and HP1 Localization in Drosophila Are Dependent on the RNAi Machinery}, url = {http://dx.doi.org/10.1126/science.1092653}, volume = {303}, year = {2004} }

TY - JOUR ID - palbhadra2004 L3 - citeulike-article-id:140166 N2 - Genes normally resident in euchromatic domains are silenced when packaged into heterochromatin, as exemplified in Drosophila melanogaster by position effect variegation (PEV). Loss-of-function mutations resulting in suppression of PEV have identified critical components of heterochromatin, including proteins HP1, HP2, and histone H3 lysine 9 methyltransferase. Here, we demonstrate that this silencing is dependent on the RNA interference machinery, using tandem mini-white arrays and white transgenes in heterochromatin to show loss of silencing as a result of mutations in piwi, aubergine, or spindle-E (homeless), which encode RNAi components. These mutations result in reduction of H3 Lys9 methylation and delocalization of HP1 and HP2, most dramatically in spindle-E mutants. 10.1126/science.1092653 IS - 5658 JF - Science SN - 1095-9203 AD - Division of Biological Sciences, 117 Tucker Hall, University of Missouri, Columbia, MO 65211, USA. EP - 672 TI - Heterochromatic Silencing and HP1 Localization in Drosophila Are Dependent on the RNAi Machinery VL - 303 SP - 669 KW - chromatin KW - drosophila KW - hp1 KW - rnai AU - Pal-Bhadra, Manika AU - Leibovitch, Boris AU - Gandhi, Sumit AU - Rao, Madhusudana AU - Bhadra, Utpal AU - Birchler, James AU - Elgin, Sarah PY - 2004/01/30/ UR - http://dx.doi.org/10.1126/science.1092653 ER -