Identification of hundreds of conserved and nonconserved human microRNAs Export

@article{citeulike:241630, abstract = {MicroRNAs are noncoding RNAs of 22 nucleotides that suppress translation of target genes by binding to their mRNA and thus have a central role in gene regulation in health and disease1, 2, 3, 4, 5. To date, 222 human microRNAs have been identified6, 86 by random cloning and sequencing, 43 by computational approaches and the rest as putative microRNAs homologous to microRNAs in other species. To prove our hypothesis that the total number of microRNAs may be much larger and that several have emerged only in primates, we developed an integrative approach combining bioinformatic predictions with microarray analysis and sequence-directed cloning. Here we report the use of this approach to clone and sequence 89 new human microRNAs (nearly doubling the current number of sequenced human microRNAs), 53 of which are not conserved beyond primates. These findings suggest that the total number of human microRNAs is at least 800.}, author = {Bentwich, Isaac and Avniel, Amir and Karov, Yael and Aharonov, Ranit and Gilad, Shlomit and Barad, Omer and Barzilai, Adi and Einat, Paz and Einav, Uri and Meiri, Eti and Sharon, Eilon and Spector, Yael and Bentwich, Zvi}, booktitle = {Nat Genet}, citeulike-article-id = {241630}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/ng1590}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/ng1590}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15965474}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15965474}, day = {19}, doi = {10.1038/ng1590}, issn = {1061-4036}, journal = {Nature Genetics}, keywords = {conservation, human, mirna}, month = {June}, number = {7}, pages = {766--770}, posted-at = {2009-07-19 20:05:36}, priority = {2}, publisher = {Nature Publishing Group}, title = {Identification of hundreds of conserved and nonconserved human microRNAs}, url = {http://dx.doi.org/10.1038/ng1590}, volume = {37}, year = {2005} }

TY - JOUR ID - citeulike:241630 L3 - citeulike-article-id:241630 N2 - MicroRNAs are noncoding RNAs of 22 nucleotides that suppress translation of target genes by binding to their mRNA and thus have a central role in gene regulation in health and disease1, 2, 3, 4, 5. To date, 222 human microRNAs have been identified6, 86 by random cloning and sequencing, 43 by computational approaches and the rest as putative microRNAs homologous to microRNAs in other species. To prove our hypothesis that the total number of microRNAs may be much larger and that several have emerged only in primates, we developed an integrative approach combining bioinformatic predictions with microarray analysis and sequence-directed cloning. Here we report the use of this approach to clone and sequence 89 new human microRNAs (nearly doubling the current number of sequenced human microRNAs), 53 of which are not conserved beyond primates. These findings suggest that the total number of human microRNAs is at least 800. IS - 7 JF - Nature Genetics SN - 1061-4036 EP - 770 TI - Identification of hundreds of conserved and nonconserved human microRNAs PB - Nature Publishing Group VL - 37 T2 - Nat Genet SP - 766 KW - conservation KW - human KW - mirna AU - Bentwich, Isaac AU - Avniel, Amir AU - Karov, Yael AU - Aharonov, Ranit AU - Gilad, Shlomit AU - Barad, Omer AU - Barzilai, Adi AU - Einat, Paz AU - Einav, Uri AU - Meiri, Eti AU - Sharon, Eilon AU - Spector, Yael AU - Bentwich, Zvi PY - 2005/06/19/ UR - http://dx.doi.org/10.1038/ng1590 ER -