RNAi Related Mechanisms Affect Both Transcriptional and Posttranscriptional Transgene Silencing in Drosophila Export

@article{palbhadra2002, abstract = {Two types of transgene silencing were found for the Alcohol dehydrogenase ( Adh ) transcription unit. Transcriptional gene silencing (TGS) is Polycomb dependent and occurs when Adh is driven by the white eye color gene promoter. Full-length Adh transgenes are silenced posttranscriptionally at high copy number or by a pulsed increase over a threshold. The posttranscriptional gene silencing (PTGS) exhibits molecular hallmarks typical of RNA interference (RNAi), including the production of 21–25 bp length sense and antisense RNAs homologous to the silenced RNA. Mutations in piwi , which belongs to a gene family with members required for RNAi, block PTGS and one aspect of TGS, indicating a connection between the two types of silencing.}, author = {Pal-Bhadra, Manika and Bhadra, Utpal and Birchler, James A.}, citeulike-article-id = {5041134}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/S1097-2765(02)00440-9}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S1097276502004409}, doi = {10.1016/S1097-2765(02)00440-9}, issn = {10972765}, journal = {Molecular Cell}, keywords = {drosophila, rnai, silencing}, month = {February}, number = {2}, pages = {315--327}, posted-at = {2009-07-02 11:05:07}, priority = {2}, title = {RNAi Related Mechanisms Affect Both Transcriptional and Posttranscriptional Transgene Silencing in Drosophila}, url = {http://dx.doi.org/10.1016/S1097-2765(02)00440-9}, volume = {9}, year = {2002} }

TY - JOUR ID - palbhadra2002 L3 - citeulike-article-id:5041134 N2 - Two types of transgene silencing were found for the Alcohol dehydrogenase ( Adh ) transcription unit. Transcriptional gene silencing (TGS) is Polycomb dependent and occurs when Adh is driven by the white eye color gene promoter. Full-length Adh transgenes are silenced posttranscriptionally at high copy number or by a pulsed increase over a threshold. The posttranscriptional gene silencing (PTGS) exhibits molecular hallmarks typical of RNA interference (RNAi), including the production of 21–25 bp length sense and antisense RNAs homologous to the silenced RNA. Mutations in piwi , which belongs to a gene family with members required for RNAi, block PTGS and one aspect of TGS, indicating a connection between the two types of silencing. IS - 2 JF - Molecular Cell SN - 10972765 EP - 327 TI - RNAi Related Mechanisms Affect Both Transcriptional and Posttranscriptional Transgene Silencing in Drosophila VL - 9 SP - 315 KW - drosophila KW - rnai KW - silencing AU - Pal-Bhadra, Manika AU - Bhadra, Utpal AU - Birchler, James PY - 2002/02// UR - http://dx.doi.org/10.1016/S1097-2765(02)00440-9 ER -