Endothelium-specific ablation of PDGFB leads to pericyte loss and glomerular, cardiac and placental abnormalities Export

@article{citeulike:2742727, abstract = {Platelet-derived growth factor-B (PDGFB) is necessary for normal cardiovascular development, but the relative importance of different cellular sources of PDGFB has not been established. Using Cre-lox techniques, we show here that genetic ablation of Pdgfb in endothelial cells leads to impaired recruitment of pericytes to blood vessels. The endothelium-restricted Pdgfb knockout mutants also developed organ defects including cardiac, placental and renal abnormalities. These defects were similar to those observed in Pdgfb null mice. However, in marked contrast to the embryonic lethality of Pdgfb null mutants, the endothelium-specific mutants survived into adulthood with persistent pathological changes, including brain microhemorrhages, focal astrogliosis, and kidney glomerulus abnormalities. This spectrum of pathological changes is reminiscent of diabetic microangiopathy, suggesting that the endothelium-restricted Pdgfb knockouts may serve as models for some of the pathogenic events of vascular complications to diabetes. 10.1242/dev.01080}, author = {Bjarnegard, Mattias and Enge, Maria and Norlin, Jenny and Gustafsdottir, Sigrun and Fredriksson, Simon and Abramsson, Alexandra and Takemoto, Minoru and Gustafsson, Erika and Fassler, Reinhard and Betsholtz, Christer}, citeulike-article-id = {2742727}, citeulike-linkout-0 = {http://dx.doi.org/10.1242/dev.01080}, citeulike-linkout-1 = {http://dev.biologists.org/cgi/content/abstract/131/8/1847}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15084468}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15084468}, day = {15}, doi = {10.1242/dev.01080}, journal = {Development}, keywords = {conditional\_ko, cre, endothelial, mouse, pdgfb, pericyte, placental}, month = {April}, number = {8}, pages = {1847--1857}, posted-at = {2008-05-01 14:56:58}, priority = {2}, title = {Endothelium-specific ablation of PDGFB leads to pericyte loss and glomerular, cardiac and placental abnormalities}, url = {http://dx.doi.org/10.1242/dev.01080}, volume = {131}, year = {2004} }

TY - JOUR ID - citeulike:2742727 L3 - citeulike-article-id:2742727 N2 - Platelet-derived growth factor-B (PDGFB) is necessary for normal cardiovascular development, but the relative importance of different cellular sources of PDGFB has not been established. Using Cre-lox techniques, we show here that genetic ablation of Pdgfb in endothelial cells leads to impaired recruitment of pericytes to blood vessels. The endothelium-restricted Pdgfb knockout mutants also developed organ defects including cardiac, placental and renal abnormalities. These defects were similar to those observed in Pdgfb null mice. However, in marked contrast to the embryonic lethality of Pdgfb null mutants, the endothelium-specific mutants survived into adulthood with persistent pathological changes, including brain microhemorrhages, focal astrogliosis, and kidney glomerulus abnormalities. This spectrum of pathological changes is reminiscent of diabetic microangiopathy, suggesting that the endothelium-restricted Pdgfb knockouts may serve as models for some of the pathogenic events of vascular complications to diabetes. 10.1242/dev.01080 IS - 8 JF - Development EP - 1857 TI - Endothelium-specific ablation of PDGFB leads to pericyte loss and glomerular, cardiac and placental abnormalities VL - 131 SP - 1847 KW - conditional_ko KW - cre KW - endothelial KW - mouse KW - pdgfb KW - pericyte KW - placental AU - Bjarnegard, Mattias AU - Enge, Maria AU - Norlin, Jenny AU - Gustafsdottir, Sigrun AU - Fredriksson, Simon AU - Abramsson, Alexandra AU - Takemoto, Minoru AU - Gustafsson, Erika AU - Fassler, Reinhard AU - Betsholtz, Christer PY - 2004/04/15/ UR - http://dx.doi.org/10.1242/dev.01080 ER -