Vascular sprout formation entails tissue deformations and VE-cadherin-dependent cell-autonomous motility Export

@article{citeulike:2742950, abstract = {Embryonic and fetal vascular sprouts form within constantly expanding tissues. Nevertheless, most biological assays of vascular spouting are conducted in a static mechanical milieu. Here we study embryonic mouse allantoides, which normally give raise to an umbilical artery and vein. However, when placed in culture, allantoides assemble a primary vascular network. Unlike other in vitro assays, allantoic primordial vascular cells are situated on the upper surface of a cellular layer that is engaged in robust spreading motion. Time-lapse imaging allows quantification of primordial vascular cell motility as well as the underlying mesothelial tissue motion. Specifically, we calculate endothelial cell-autonomous motion by subtracting the tissue-level mesothelial motion from the total endothelial cell displacements. Formation of new vascular polygons is hindered by administration of function-blocking VE-cadherin antibodies. Time-lapse recordings reveal that (1) cells at the base of sprouts normally move distally "over" existing sprout cells to form new tip-cells; and (2) loss of VE-cadherin activity prevents this motile behavior. Thus, endothelial cell-cell-adhesion-based motility is required for the advancement of vascular sprouts within a moving tissue environment. To the best of our knowledge, this is the first study that couples endogenous tissue dynamics to assembly of vascular networks in a mammalian system.}, author = {Perryn, Erica D. and Czir\'{o}k, Andr\'{a}s and Little, Charles D.}, citeulike-article-id = {2742950}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.ydbio.2007.10.036}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0012160607014819}, citeulike-linkout-2 = {http://www.sciencedirect.com/science/article/B6WDG-4R29FNM-3/1/b637bf220416bc579b0cb2cde29d43a4}, day = {15}, doi = {10.1016/j.ydbio.2007.10.036}, journal = {Developmental Biology}, keywords = {allantoid, allantois, ve-cadherin}, month = {January}, number = {2}, pages = {545--555}, posted-at = {2008-05-01 16:32:09}, priority = {2}, title = {Vascular sprout formation entails tissue deformations and VE-cadherin-dependent cell-autonomous motility}, url = {http://dx.doi.org/10.1016/j.ydbio.2007.10.036}, volume = {313}, year = {2008} }

TY - JOUR ID - citeulike:2742950 L3 - citeulike-article-id:2742950 N2 - Embryonic and fetal vascular sprouts form within constantly expanding tissues. Nevertheless, most biological assays of vascular spouting are conducted in a static mechanical milieu. Here we study embryonic mouse allantoides, which normally give raise to an umbilical artery and vein. However, when placed in culture, allantoides assemble a primary vascular network. Unlike other in vitro assays, allantoic primordial vascular cells are situated on the upper surface of a cellular layer that is engaged in robust spreading motion. Time-lapse imaging allows quantification of primordial vascular cell motility as well as the underlying mesothelial tissue motion. Specifically, we calculate endothelial cell-autonomous motion by subtracting the tissue-level mesothelial motion from the total endothelial cell displacements. Formation of new vascular polygons is hindered by administration of function-blocking VE-cadherin antibodies. Time-lapse recordings reveal that (1) cells at the base of sprouts normally move distally "over" existing sprout cells to form new tip-cells; and (2) loss of VE-cadherin activity prevents this motile behavior. Thus, endothelial cell-cell-adhesion-based motility is required for the advancement of vascular sprouts within a moving tissue environment. To the best of our knowledge, this is the first study that couples endogenous tissue dynamics to assembly of vascular networks in a mammalian system. IS - 2 JF - Developmental Biology EP - 555 TI - Vascular sprout formation entails tissue deformations and VE-cadherin-dependent cell-autonomous motility VL - 313 SP - 545 KW - allantoid KW - allantois KW - ve-cadherin AU - Perryn, Erica AU - Czirók, András AU - Little, Charles PY - 2008/01/15/ UR - http://dx.doi.org/10.1016/j.ydbio.2007.10.036 ER -