VE-cadherin-CreERT2 transgenic mouse: a model for inducible recombination in the endothelium. Export

@article{citeulike:2742998, abstract = {To introduce temporal control in genetic experiments targeting the endothelium, we established a mouse line expressing tamoxifen-inducible Cre-recombinase (Cre-ERT2) under the regulation of the vascular endothelial cadherin promoter (VECad). Specificity and efficiency of Cre activity was documented by crossing VECad-Cre-ERT2 with the ROSA26R reporter mouse, in which a floxed-stop cassette has been placed upstream of the beta-galactosidase gene. We found that tamoxifen specifically induced widespread recombination in the endothelium of embryonic, neonatal, and adult tissues. Recombination was also documented in tumor-associated vascular beds and in postnatal angiogenesis assays. Furthermore, injection of tamoxifen in adult animals resulted in negligible excision (lower than 0.4\%) in the hematopoietic lineage. The VECad-Cre-ERT2 mouse is likely to be a valuable tool to study the function of genes involved in vascular development, homeostasis, and in complex processes involving neoangiogenesis, such as tumor growth.}, address = {Department of Molecular Cellular and Developmental Biology, UCLA, Los Angeles, California 90095, USA.}, author = {Monvoisin, A. and Alva, J. A. and Hofmann, J. J. and Zovein, A. C. and Lane, T. F. and Iruela-Arispe, M. L.}, citeulike-article-id = {2742998}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/dvdy.20982}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17072878}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17072878}, doi = {10.1002/dvdy.20982}, issn = {1058-8388}, journal = {Developmental dynamics : an official publication of the American Association of Anatomists}, keywords = {cre, ert-2, ve-cadherin}, month = {December}, number = {12}, pages = {3413--3422}, posted-at = {2008-05-01 16:56:41}, priority = {2}, title = {VE-cadherin-CreERT2 transgenic mouse: a model for inducible recombination in the endothelium.}, url = {http://dx.doi.org/10.1002/dvdy.20982}, volume = {235}, year = {2006} }

TY - JOUR ID - citeulike:2742998 L3 - citeulike-article-id:2742998 N2 - To introduce temporal control in genetic experiments targeting the endothelium, we established a mouse line expressing tamoxifen-inducible Cre-recombinase (Cre-ERT2) under the regulation of the vascular endothelial cadherin promoter (VECad). Specificity and efficiency of Cre activity was documented by crossing VECad-Cre-ERT2 with the ROSA26R reporter mouse, in which a floxed-stop cassette has been placed upstream of the beta-galactosidase gene. We found that tamoxifen specifically induced widespread recombination in the endothelium of embryonic, neonatal, and adult tissues. Recombination was also documented in tumor-associated vascular beds and in postnatal angiogenesis assays. Furthermore, injection of tamoxifen in adult animals resulted in negligible excision (lower than 0.4%) in the hematopoietic lineage. The VECad-Cre-ERT2 mouse is likely to be a valuable tool to study the function of genes involved in vascular development, homeostasis, and in complex processes involving neoangiogenesis, such as tumor growth. IS - 12 JF - Developmental dynamics : an official publication of the American Association of Anatomists SN - 1058-8388 AD - Department of Molecular Cellular and Developmental Biology, UCLA, Los Angeles, California 90095, USA. EP - 3422 TI - VE-cadherin-CreERT2 transgenic mouse: a model for inducible recombination in the endothelium. VL - 235 SP - 3413 KW - cre KW - ert-2 KW - ve-cadherin AU - Monvoisin, A AU - Alva, JA AU - Hofmann, JJ AU - Zovein, AC AU - Lane, TF AU - Iruela-Arispe, ML PY - 2006/12// UR - http://dx.doi.org/10.1002/dvdy.20982 ER -