RhoA and Rac mediate endothelial cell polarization and detachment induced by T-cadherin. Export

@article{citeulike:4862476, abstract = {T-cadherin (T-cad) is an atypical GPI-anchored member of the cadherin superfamily. Ligation of T-cad receptors on endothelial cells prevents cell spreading, promotes elongation and polarization, decreases adhesion to the matrix, and facilitates migration. This study investigates involvement of Rho GTPases in T-cad signaling. Human umbilical vein endothelial cells were infected with adenoviral vectors expressing dominant-negative and/or constitutively active mutants of RhoA (N19RhoA/RhoA63), ROCK (RB/PH(TT)/CAT), and Rac1 (N17RAC). Mutant-infected and empty vector-infected cells were compared with respect to their ability to detach and polarize when plated on substratum containing recombinant T-cad protein used as a ligand mimicking homophilic T-cad interactions. ROCK involvement was also studied using specific inhibitor Y-27632. Adhesion assays, analysis of cell phenotype, and actin cytoskeleton organization using TRITC-labeled phalloidin demonstrated that T-cad-induced cell polarization includes two complementary components: RhoA/ROCK pathway is necessary for cell contraction, stress fiber assembly, and inhibition of spreading, whereas Rac is required for formation of actin-rich lamellipodia at the leading edges of polarized cells. Individual repression of either pathway only partially prevented cell polarization and detachment, while simultaneous repression of RhoA and Rac pathways fully eliminated responses to homophilic T-cad ligation. In conclusion, these data suggest that T-cad induces cell deadhesion and polarization via RhoA-ROCK- and Rac-dependent mechanisms.}, author = {Philippova, M. and Ivanov, D. and Allenspach, R. and Takuwa, Y. and Erne, P. and Resink, T.}, citeulike-article-id = {4862476}, citeulike-linkout-0 = {http://dx.doi.org/10.1096/fj.04-2430fje}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15703273}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15703273}, doi = {10.1096/fj.04-2430fje}, issn = {1530-6860}, journal = {The FASEB journal : official publication of the Federation of American Societies for Experimental Biology}, keywords = {huvec, inhibitor, migration, polarization, rac, rhoa, rock, t-cadherin}, month = {April}, number = {6}, pages = {588--590}, posted-at = {2009-06-15 23:00:14}, priority = {2}, title = {RhoA and Rac mediate endothelial cell polarization and detachment induced by T-cadherin.}, url = {http://dx.doi.org/10.1096/fj.04-2430fje}, volume = {19}, year = {2005} }

TY - JOUR ID - citeulike:4862476 L3 - citeulike-article-id:4862476 N2 - T-cadherin (T-cad) is an atypical GPI-anchored member of the cadherin superfamily. Ligation of T-cad receptors on endothelial cells prevents cell spreading, promotes elongation and polarization, decreases adhesion to the matrix, and facilitates migration. This study investigates involvement of Rho GTPases in T-cad signaling. Human umbilical vein endothelial cells were infected with adenoviral vectors expressing dominant-negative and/or constitutively active mutants of RhoA (N19RhoA/RhoA63), ROCK (RB/PH(TT)/CAT), and Rac1 (N17RAC). Mutant-infected and empty vector-infected cells were compared with respect to their ability to detach and polarize when plated on substratum containing recombinant T-cad protein used as a ligand mimicking homophilic T-cad interactions. ROCK involvement was also studied using specific inhibitor Y-27632. Adhesion assays, analysis of cell phenotype, and actin cytoskeleton organization using TRITC-labeled phalloidin demonstrated that T-cad-induced cell polarization includes two complementary components: RhoA/ROCK pathway is necessary for cell contraction, stress fiber assembly, and inhibition of spreading, whereas Rac is required for formation of actin-rich lamellipodia at the leading edges of polarized cells. Individual repression of either pathway only partially prevented cell polarization and detachment, while simultaneous repression of RhoA and Rac pathways fully eliminated responses to homophilic T-cad ligation. In conclusion, these data suggest that T-cad induces cell deadhesion and polarization via RhoA-ROCK- and Rac-dependent mechanisms. IS - 6 JF - The FASEB journal : official publication of the Federation of American Societies for Experimental Biology SN - 1530-6860 EP - 590 TI - RhoA and Rac mediate endothelial cell polarization and detachment induced by T-cadherin. VL - 19 SP - 588 KW - huvec KW - inhibitor KW - migration KW - polarization KW - rac KW - rhoa KW - rock KW - t-cadherin AU - Philippova, M AU - Ivanov, D AU - Allenspach, R AU - Takuwa, Y AU - Erne, P AU - Resink, T PY - 2005/04// UR - http://dx.doi.org/10.1096/fj.04-2430fje ER -