Filtering of deep sequencing data reveals the existence of abundant Dicer-dependent small RNAs derived from tRNAs Export

@article{citeulike:6111181, abstract = {10.1261/rna.1738409 Deep sequencing technologies such as Illumina, SOLiD, and 454 platforms have become very powerful tools in discovering and quantifying small RNAs in diverse organisms. Sequencing small RNA fractions always identifies RNAs derived from abundant RNA species such as rRNAs, tRNAs, snRNA, and snoRNA, and they are widely considered to be random degradation products. We carried out bioinformatic analysis of deep sequenced HeLa RNA and after quality filtering, identified highly abundant small RNA fragments, derived from mature tRNAs that are likely produced by specific processing rather than from random degradation. Moreover, we showed that the processing of small RNAs derived from tRNA is dependent on Dicer in vivo and that Dicer cleaves the tRNA in vitro.}, author = {Cole, Christian and Sobala, Andrew and Lu, Cheng and Thatcher, Shawn R. and Bowman, Andrew and Brown, John W. S. and Green, Pamela J. and Barton, Geoffrey J. and Hutvagner, Gyorgy}, citeulike-article-id = {6111181}, citeulike-linkout-0 = {http://dx.doi.org/10.1261/rna.1738409}, citeulike-linkout-1 = {http://rnajournal.cshlp.org/content/15/12/2147.abstract}, citeulike-linkout-2 = {http://rnajournal.cshlp.org/content/15/12/2147.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19850906}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19850906}, day = {22}, doi = {10.1261/rna.1738409}, issn = {1469-9001}, journal = {RNA}, keywords = {dicer}, month = {December}, number = {12}, pages = {2147--2160}, posted-at = {2009-11-13 22:46:39}, priority = {2}, title = {Filtering of deep sequencing data reveals the existence of abundant Dicer-dependent small RNAs derived from tRNAs}, url = {http://dx.doi.org/10.1261/rna.1738409}, volume = {15}, year = {2009} }

TY - JOUR ID - citeulike:6111181 L3 - citeulike-article-id:6111181 N2 - 10.1261/rna.1738409 Deep sequencing technologies such as Illumina, SOLiD, and 454 platforms have become very powerful tools in discovering and quantifying small RNAs in diverse organisms. Sequencing small RNA fractions always identifies RNAs derived from abundant RNA species such as rRNAs, tRNAs, snRNA, and snoRNA, and they are widely considered to be random degradation products. We carried out bioinformatic analysis of deep sequenced HeLa RNA and after quality filtering, identified highly abundant small RNA fragments, derived from mature tRNAs that are likely produced by specific processing rather than from random degradation. Moreover, we showed that the processing of small RNAs derived from tRNA is dependent on Dicer in vivo and that Dicer cleaves the tRNA in vitro. IS - 12 JF - RNA SN - 1469-9001 EP - 2160 TI - Filtering of deep sequencing data reveals the existence of abundant Dicer-dependent small RNAs derived from tRNAs VL - 15 SP - 2147 KW - dicer AU - Cole, Christian AU - Sobala, Andrew AU - Lu, Cheng AU - Thatcher, Shawn AU - Bowman, Andrew AU - Brown, John AU - Green, Pamela AU - Barton, Geoffrey AU - Hutvagner, Gyorgy PY - 2009/12/22/ UR - http://dx.doi.org/10.1261/rna.1738409 ER -