GDNF selectively promotes regeneration of injury-primed sensory neurons in the lesioned spinal cord. Export

@article{citeulike:2090678, abstract = {Axonal regeneration within the CNS fails due to the growth inhibitory environment and the limited intrinsic growth capacity of injured neurons. Injury to DRG peripheral axons induces expression of growth associated genes including members of the glial-derived neurotrophic factor (GDNF) signaling pathway and "preconditions" the injured cells into an active growth state, enhancing growth of their centrally projecting axons. Here, we show that preconditioning DRG neurons prior to culturing increased neurite outgrowth, which was further enhanced by GDNF in a bell-shaped growth response curve. In vivo, GDNF delivered directly to DRG cell bodies facilitated the preconditioning effect, further enhancing axonal regeneration beyond spinal cord lesions. Consistent with the in vitro results, the in vivo effect was seen only at low GDNF concentrations. We conclude that peripheral nerve injury upregulates GDNF signaling pathway components and that exogenous GDNF treatment selectively promotes axonal growth of injury-primed sensory neurons in a concentration-dependent fashion.}, address = {Neural Plasticity Research Group, Massachusetts General Hospital and Harvard Medical School, 149 13th Street, Charlestown, MA 02129, USA.}, author = {Mills, C. D. and Allchorne, A. J. and Griffin, R. S. and Woolf, C. J. and Costigan, M.}, citeulike-article-id = {2090678}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.mcn.2007.06.011}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17702601}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17702601}, doi = {10.1016/j.mcn.2007.06.011}, issn = {1044-7431}, journal = {Mol Cell Neurosci}, keywords = {bdnf, drg}, month = {October}, number = {2}, pages = {185--194}, posted-at = {2007-12-11 16:40:51}, priority = {2}, title = {GDNF selectively promotes regeneration of injury-primed sensory neurons in the lesioned spinal cord.}, url = {http://dx.doi.org/10.1016/j.mcn.2007.06.011}, volume = {36}, year = {2007} }

TY - JOUR ID - citeulike:2090678 L3 - citeulike-article-id:2090678 N2 - Axonal regeneration within the CNS fails due to the growth inhibitory environment and the limited intrinsic growth capacity of injured neurons. Injury to DRG peripheral axons induces expression of growth associated genes including members of the glial-derived neurotrophic factor (GDNF) signaling pathway and "preconditions" the injured cells into an active growth state, enhancing growth of their centrally projecting axons. Here, we show that preconditioning DRG neurons prior to culturing increased neurite outgrowth, which was further enhanced by GDNF in a bell-shaped growth response curve. In vivo, GDNF delivered directly to DRG cell bodies facilitated the preconditioning effect, further enhancing axonal regeneration beyond spinal cord lesions. Consistent with the in vitro results, the in vivo effect was seen only at low GDNF concentrations. We conclude that peripheral nerve injury upregulates GDNF signaling pathway components and that exogenous GDNF treatment selectively promotes axonal growth of injury-primed sensory neurons in a concentration-dependent fashion. IS - 2 JF - Mol Cell Neurosci SN - 1044-7431 AD - Neural Plasticity Research Group, Massachusetts General Hospital and Harvard Medical School, 149 13th Street, Charlestown, MA 02129, USA. EP - 194 TI - GDNF selectively promotes regeneration of injury-primed sensory neurons in the lesioned spinal cord. VL - 36 SP - 185 KW - bdnf KW - drg AU - Mills, CD AU - Allchorne, AJ AU - Griffin, RS AU - Woolf, CJ AU - Costigan, M PY - 2007/10// UR - http://dx.doi.org/10.1016/j.mcn.2007.06.011 ER -