Vasoactive intestinal peptide influences neurite outgrowth in cultured rat spinal cord neurons. Export

@article{citeulike:2309994, abstract = {Vasoactive intestinal peptide (VIP) is a neuropeptide which has been shown to exhibit a wide range of neurotrophic effects both in vivo and in vitro. For the purpose of clarifying the effect of VIP on spinal cord neurons, we studied the effect of VIP on neurite outgrowth of fetal rat ventral and dorsal portions of spinal cord in cultures. VIP-treated ventral spinal cord cultures (VSCC), compared with control VSCC, had a significant neurite outgrowth at 10(-8), 10(-6), and 10(-4) M. The effect was considered to be concentration dependent. Morphological changes of the dorsal spinal cord cultures (DSCC) remained unchanged by VIP treatment. Because of their close sequence homology with VIP, PHI-27 (peptide, histidylisoleucine amide) and secretin were also examined with the same experimental conditions as was VIP. Both PHI-27 and secretin had neurite promoting effects in VSCC at 10(-8) and 10(-6) M, respectively. However, there were no neurite promoting effects in DSCC in both of them at any concentrations. VIP had the most potent effect on neurite outgrowth in VSCC, followed by PHI-27, and secretin in their effectiveness concentrations. Our data showing VIP, PHI-27 and secretin have neurotrophic action on VSCC and suggest that a potential therapeutic use of VIP and its related peptides in treating diseases that involve degeneration and death of spinal motor neurons, such as motor neuropathy and amyotrophic lateral sclerosis.}, address = {The Fourth Department of Internal Medicine, Toho University Ohashi Hospital, Tokyo, Japan. yaso@med.toho-u.ac.jp}, author = {Iwasaki, Y. and Ikeda, K. and Ichikawa, Y. and Igarashi, O.}, citeulike-article-id = {2309994}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/11760877}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=11760877}, issn = {0161-6412}, journal = {Neurol Res}, keywords = {drg}, month = {December}, number = {8}, pages = {851--854}, posted-at = {2008-01-31 08:56:51}, priority = {2}, title = {Vasoactive intestinal peptide influences neurite outgrowth in cultured rat spinal cord neurons.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/11760877}, volume = {23}, year = {2001} }

TY - JOUR ID - citeulike:2309994 L3 - citeulike-article-id:2309994 N2 - Vasoactive intestinal peptide (VIP) is a neuropeptide which has been shown to exhibit a wide range of neurotrophic effects both in vivo and in vitro. For the purpose of clarifying the effect of VIP on spinal cord neurons, we studied the effect of VIP on neurite outgrowth of fetal rat ventral and dorsal portions of spinal cord in cultures. VIP-treated ventral spinal cord cultures (VSCC), compared with control VSCC, had a significant neurite outgrowth at 10(-8), 10(-6), and 10(-4) M. The effect was considered to be concentration dependent. Morphological changes of the dorsal spinal cord cultures (DSCC) remained unchanged by VIP treatment. Because of their close sequence homology with VIP, PHI-27 (peptide, histidylisoleucine amide) and secretin were also examined with the same experimental conditions as was VIP. Both PHI-27 and secretin had neurite promoting effects in VSCC at 10(-8) and 10(-6) M, respectively. However, there were no neurite promoting effects in DSCC in both of them at any concentrations. VIP had the most potent effect on neurite outgrowth in VSCC, followed by PHI-27, and secretin in their effectiveness concentrations. Our data showing VIP, PHI-27 and secretin have neurotrophic action on VSCC and suggest that a potential therapeutic use of VIP and its related peptides in treating diseases that involve degeneration and death of spinal motor neurons, such as motor neuropathy and amyotrophic lateral sclerosis. IS - 8 JF - Neurol Res SN - 0161-6412 AD - The Fourth Department of Internal Medicine, Toho University Ohashi Hospital, Tokyo, Japan. yaso@med.toho-u.ac.jp EP - 854 TI - Vasoactive intestinal peptide influences neurite outgrowth in cultured rat spinal cord neurons. VL - 23 SP - 851 KW - drg AU - Iwasaki, Y AU - Ikeda, K AU - Ichikawa, Y AU - Igarashi, O PY - 2001/12// UR - http://view.ncbi.nlm.nih.gov/pubmed/11760877 ER -