MicroRNA expression profiles for the NCI-60 cancer cell panel Export

@article{Blower2007, abstract = {10.1158/1535-7163.MCT-07-0009 Advances in the understanding of cancer cell biology and response to drug treatment have benefited from new molecular technologies and methods for integrating information from multiple sources. The NCI-60, a panel of 60 diverse human cancer cell lines, has been used by the National Cancer Institute to screen >100,000 chemical compounds and natural product extracts for anticancer activity. The NCI-60 has also been profiled for mRNA and protein expression, mutational status, chromosomal aberrations, and DNA copy number, generating an unparalleled public resource for integrated chemogenomic studies. Recently, microRNAs have been shown to target particular sets of mRNAs, thereby preventing translation or accelerating mRNA turnover. To complement the existing NCI-60 data sets, we have measured expression levels of microRNAs in the NCI-60 and incorporated the resulting data into the CellMiner program package for integrative analysis. Cell line groupings based on microRNA expression were generally consistent with tissue type and with cell line clustering based on mRNA expression. However, mRNA expression seemed to be somewhat more informative for discriminating among tissue types than was microRNA expression. In addition, we found that there does not seem to be a significant correlation between microRNA expression patterns and those of known target transcripts. Comparison of microRNA expression patterns and compound potency patterns showed significant correlations, suggesting that microRNAs may play a role in chemoresistance. Combined with gene expression and other biological data using multivariate analysis, microRNA expression profiles may provide a critical link for understanding mechanisms involved in chemosensitivity and chemoresistance. [Mol Cancer Ther 2007;6(5):1483\^{a}€“91]}, author = {Blower, Paul E. and Verducci, Joseph S. and Lin, Shili and Zhou, Jin and Chung, Ji-Hyun and Dai, Zunyan and Liu, Chang-Gong and Reinhold, William and Lorenzi, Philip L. and Kaldjian, Eric P. and Croce, Carlo M. and Weinstein, John N. and Sadee, Wolfgang}, citeulike-article-id = {1674824}, citeulike-linkout-0 = {http://dx.doi.org/10.1158/1535-7163.MCT-07-0009}, citeulike-linkout-1 = {http://mct.aacrjournals.org/content/6/5/1483.abstract}, citeulike-linkout-2 = {http://mct.aacrjournals.org/content/6/5/1483.full.pdf}, citeulike-linkout-3 = {http://mct.aacrjournals.org/cgi/content/abstract/6/5/1483}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/17483436}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=17483436}, day = {1}, doi = {10.1158/1535-7163.MCT-07-0009}, journal = {Molecular Cancer Therapeutics}, keywords = {mirna\_and\_nci60, nci60}, month = {May}, number = {5}, pages = {1483--1491}, posted-at = {2009-11-07 13:18:51}, priority = {2}, title = {MicroRNA expression profiles for the NCI-60 cancer cell panel}, url = {http://dx.doi.org/10.1158/1535-7163.MCT-07-0009}, volume = {6}, year = {2007} }

TY - JOUR ID - Blower2007 L3 - citeulike-article-id:1674824 N2 - 10.1158/1535-7163.MCT-07-0009 Advances in the understanding of cancer cell biology and response to drug treatment have benefited from new molecular technologies and methods for integrating information from multiple sources. The NCI-60, a panel of 60 diverse human cancer cell lines, has been used by the National Cancer Institute to screen >100,000 chemical compounds and natural product extracts for anticancer activity. The NCI-60 has also been profiled for mRNA and protein expression, mutational status, chromosomal aberrations, and DNA copy number, generating an unparalleled public resource for integrated chemogenomic studies. Recently, microRNAs have been shown to target particular sets of mRNAs, thereby preventing translation or accelerating mRNA turnover. To complement the existing NCI-60 data sets, we have measured expression levels of microRNAs in the NCI-60 and incorporated the resulting data into the CellMiner program package for integrative analysis. Cell line groupings based on microRNA expression were generally consistent with tissue type and with cell line clustering based on mRNA expression. However, mRNA expression seemed to be somewhat more informative for discriminating among tissue types than was microRNA expression. In addition, we found that there does not seem to be a significant correlation between microRNA expression patterns and those of known target transcripts. Comparison of microRNA expression patterns and compound potency patterns showed significant correlations, suggesting that microRNAs may play a role in chemoresistance. Combined with gene expression and other biological data using multivariate analysis, microRNA expression profiles may provide a critical link for understanding mechanisms involved in chemosensitivity and chemoresistance. [Mol Cancer Ther 2007;6(5):1483–91] IS - 5 JF - Molecular Cancer Therapeutics EP - 1491 TI - MicroRNA expression profiles for the NCI-60 cancer cell panel VL - 6 SP - 1483 KW - mirna_and_nci60 KW - nci60 AU - Blower, Paul AU - Verducci, Joseph AU - Lin, Shili AU - Zhou, Jin AU - Chung, Ji-Hyun AU - Dai, Zunyan AU - Liu, Chang-Gong AU - Reinhold, William AU - Lorenzi, Philip AU - Kaldjian, Eric AU - Croce, Carlo AU - Weinstein, John AU - Sadee, Wolfgang PY - 2007/05/01/ UR - http://dx.doi.org/10.1158/1535-7163.MCT-07-0009 ER -