Fibrils connect microtubule tips with kinetochores: a mechanism to couple tubulin dynamics to chromosome motion. Export

@article{mcintoshCell08, abstract = {Kinetochores of mitotic chromosomes are coupled to spindle microtubules in ways that allow the energy from tubulin dynamics to drive chromosome motion. Most kinetochore-associated microtubule ends display curving "protofilaments," strands of tubulin dimers that bend away from the microtubule axis. Both a kinetochore "plate" and an encircling, ring-shaped protein complex have been proposed to link protofilament bending to poleward chromosome motion. Here we show by electron tomography that slender fibrils connect curved protofilaments directly to the inner kinetochore. Fibril-protofilament associations correlate with a local straightening of the flared protofilaments. Theoretical analysis reveals that protofilament-fibril connections would be efficient couplers for chromosome motion, and experimental work on two very different kinetochore components suggests that filamentous proteins can couple shortening microtubules to cargo movements. These analyses define a ring-independent mechanism for harnessing microtubule dynamics directly to chromosome movement.}, author = {McIntosh, J. R. and Grishchuk, E. L. and Morphew, M. K. and Efremov, A. K. and Zhudenkov, K. and Volkov, V. A. and Cheeseman, I. M. and Desai, A. and Mastronarde, D. N. and Ataullakhanov, F. I.}, citeulike-article-id = {3480813}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.cell.2008.08.038}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18957206}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18957206}, day = {17}, doi = {10.1016/j.cell.2008.08.038}, issn = {1097-4172}, journal = {Cell}, keywords = {kinetochores, microtubules}, month = {October}, number = {2}, pages = {322--333}, posted-at = {2008-11-04 20:50:24}, priority = {2}, title = {Fibrils connect microtubule tips with kinetochores: a mechanism to couple tubulin dynamics to chromosome motion.}, url = {http://dx.doi.org/10.1016/j.cell.2008.08.038}, volume = {135}, year = {2008} }

TY - JOUR ID - mcintoshCell08 L3 - citeulike-article-id:3480813 N2 - Kinetochores of mitotic chromosomes are coupled to spindle microtubules in ways that allow the energy from tubulin dynamics to drive chromosome motion. Most kinetochore-associated microtubule ends display curving "protofilaments," strands of tubulin dimers that bend away from the microtubule axis. Both a kinetochore "plate" and an encircling, ring-shaped protein complex have been proposed to link protofilament bending to poleward chromosome motion. Here we show by electron tomography that slender fibrils connect curved protofilaments directly to the inner kinetochore. Fibril-protofilament associations correlate with a local straightening of the flared protofilaments. Theoretical analysis reveals that protofilament-fibril connections would be efficient couplers for chromosome motion, and experimental work on two very different kinetochore components suggests that filamentous proteins can couple shortening microtubules to cargo movements. These analyses define a ring-independent mechanism for harnessing microtubule dynamics directly to chromosome movement. IS - 2 JF - Cell SN - 1097-4172 EP - 333 TI - Fibrils connect microtubule tips with kinetochores: a mechanism to couple tubulin dynamics to chromosome motion. VL - 135 SP - 322 KW - kinetochores KW - microtubules AU - McIntosh, JR AU - Grishchuk, EL AU - Morphew, MK AU - Efremov, AK AU - Zhudenkov, K AU - Volkov, VA AU - Cheeseman, IM AU - Desai, A AU - Mastronarde, DN AU - Ataullakhanov, FI PY - 2008/10/17/ UR - http://dx.doi.org/10.1016/j.cell.2008.08.038 ER -