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Molecular Breeding, Vol. 23, No. 2. (1 February 2009), pp. 337-347, doi:10.1007/s11032-008-9246-7 Key: citeulike:3784536
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The development of high-oleate soybean germplasm is hindered by the lack of knowledge of the genetic factors controlling oleate phenotypic variation. In the present study, several candidate genes implicated in oleate biosynthesis were mapped and their cosegregation with oleate, linoleate and linolenate quantitative trait loci (QTLs) was investigated. FAD2-2C , a previously described ω-6 desaturase isoform, was localized on linkage group E; whereas, a novel FAD2-2 isoform, designated as FAD2-2D , mapped on linkage group N. In addition, two isoforms were identified for the aminoalcoholphosphotransferase-encoding GmAAPT1 gene, denoted AAPT1a and AAPT1b . A database query suggested that only one functional copy of the FAD6 gene, encoding a plastid localized ω-6 desaturase, exists in the soybean genome. AAPT1a and FAD6 mapped on linkage group D1b, 23.40 cM apart. Linolenate QTLs with minor effects were identified near the FAD6 and AAPT1a markers in two segregating populations.
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