High tandem repeat content in the genome of the short-lived annual fish Nothobranchius furzeri: a new vertebrate model for aging research Export

@article{citeulike:4040947, abstract = {BACKGROUND:The annual fish Nothobranchius furzeri is the vertebrate with the shortest known life span in captivity. Fish of the GRZ strain live only three to four months under optimal laboratory conditions, show explosive growth, early sexual maturation and age-dependent physiological and behavioral decline, and express aging related biomarkers. Treatment with resveratrol and low temperature significantly extends the maximum life span. These features make N. furzeri a promising new vertebrate model for age research.RESULTS:To contribute to establishing N. furzeri as a new model organism, we provide a first insight into its genome and a comparison to medaka, stickleback, tetraodon and zebrafish. The N. furzeri genome contains 19 chromosomes (2n = 38). Its genome of between 1.6 and 1.9 Gb is the largest among the analyzed fish species and has, at 45\%, the highest repeat content. Remarkably, tandem repeats comprise 21\%, which is 4-12 times more than in the other four fish species. In addition, G+C-rich tandem repeats preferentially localize to centromeric regions. Phylogenetic analysis based on coding sequences identifies medaka as the closest relative. Genotyping of an initial set of 27 markers and multi-locus fingerprinting of one microsatellite provides the first molecular evidence that the GRZ strain is highly inbred.CONCLUSIONS:Our work presents a first basis for systematic genomic and genetic analyses aimed at understanding the mechanisms of life span determination in N. furzeri.}, author = {Reichwald, Kathrin and Lauber, Chris and Nanda, Indrajit and Kirschner, Jeanette and Hartmann, Nils and Schories, Susanne and Gausmann, Ulrike and Taudien, Stefan and Schilhabel, Markus and Szafranski, Karol and Glockner, Gernot and Schmid, Michael and Cellerino, Alessandro and Schartl, Manfred and Englert, Christoph and Platzer, Matthias}, citeulike-article-id = {4040947}, citeulike-linkout-0 = {http://dx.doi.org/10.1186/gb-2009-10-2-r16}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19210790}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19210790}, day = {11}, doi = {10.1186/gb-2009-10-2-r16}, issn = {1465-6906}, journal = {Genome Biology}, keywords = {aging, model, organism, reserach}, month = {February}, number = {2}, pages = {R16+}, posted-at = {2009-09-15 23:49:43}, priority = {5}, title = {High tandem repeat content in the genome of the short-lived annual fish Nothobranchius furzeri: a new vertebrate model for aging research}, url = {http://dx.doi.org/10.1186/gb-2009-10-2-r16}, volume = {10}, year = {2009} }

TY - JOUR ID - citeulike:4040947 L3 - citeulike-article-id:4040947 N2 - BACKGROUND:The annual fish Nothobranchius furzeri is the vertebrate with the shortest known life span in captivity. Fish of the GRZ strain live only three to four months under optimal laboratory conditions, show explosive growth, early sexual maturation and age-dependent physiological and behavioral decline, and express aging related biomarkers. Treatment with resveratrol and low temperature significantly extends the maximum life span. These features make N. furzeri a promising new vertebrate model for age research.RESULTS:To contribute to establishing N. furzeri as a new model organism, we provide a first insight into its genome and a comparison to medaka, stickleback, tetraodon and zebrafish. The N. furzeri genome contains 19 chromosomes (2n = 38). Its genome of between 1.6 and 1.9 Gb is the largest among the analyzed fish species and has, at 45%, the highest repeat content. Remarkably, tandem repeats comprise 21%, which is 4-12 times more than in the other four fish species. In addition, G+C-rich tandem repeats preferentially localize to centromeric regions. Phylogenetic analysis based on coding sequences identifies medaka as the closest relative. Genotyping of an initial set of 27 markers and multi-locus fingerprinting of one microsatellite provides the first molecular evidence that the GRZ strain is highly inbred.CONCLUSIONS:Our work presents a first basis for systematic genomic and genetic analyses aimed at understanding the mechanisms of life span determination in N. furzeri. IS - 2 JF - Genome Biology SN - 1465-6906 TI - High tandem repeat content in the genome of the short-lived annual fish Nothobranchius furzeri: a new vertebrate model for aging research VL - 10 SP - R16 KW - aging KW - model KW - organism KW - reserach AU - Reichwald, Kathrin AU - Lauber, Chris AU - Nanda, Indrajit AU - Kirschner, Jeanette AU - Hartmann, Nils AU - Schories, Susanne AU - Gausmann, Ulrike AU - Taudien, Stefan AU - Schilhabel, Markus AU - Szafranski, Karol AU - Glockner, Gernot AU - Schmid, Michael AU - Cellerino, Alessandro AU - Schartl, Manfred AU - Englert, Christoph AU - Platzer, Matthias PY - 2009/02/11/ UR - http://dx.doi.org/10.1186/gb-2009-10-2-r16 ER -