Kinetic evaluation of photosensitivity in genetically engineered neurons expressing green algae light-gated channels. Export

@article{citeulike:409361, abstract = {Neurons become photosensitive by genetically introducing one of green algae-derived protein, channelrhodopsin-2 (ChR2). Here, we quantitatively investigated the rapidness of the light-gated current of ChR2 expressed in PC12 cells using blue light-emitting diode (LED) light. The light-gated current consists of two components, inactivating and non-inactivating. The magnitude of inactivating component was almost linearly related to the light intensity. The non-inactivating component showed a tendency to saturate at high illumination. Both the activation and inactivation rates of the light-gated current were linearly dependent on the light intensity. However, the activation rate (turning-on rate) is about 10-fold faster than the inactivation rate. Although the turning-off time constant was little dependent on the light intensity, that at the end of 1s light pulse was about two-fold larger than that at 20ms. Neurons are also made photosensitive by the expression of ChR2 in the living animal. Since both the turning-on and turning-off time constants of light-gated current was smaller than the membrane time constant of neurons, the LED light illumination of the photosensitive neurons was enough to evoke action potentials in a pulse-to-pulse manner in an acute slice of hippocampus.}, address = {Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan.}, author = {Ishizuka, Toru and Kakuda, Masaaki and Araki, Rikita and Yawo, Hiromu}, citeulike-article-id = {409361}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.neures.2005.10.009}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/16298005}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=16298005}, day = {16}, doi = {10.1016/j.neures.2005.10.009}, issn = {0168-0102}, journal = {Neurosci Res}, month = {November}, posted-at = {2007-09-27 11:38:20}, priority = {0}, title = {Kinetic evaluation of photosensitivity in genetically engineered neurons expressing green algae light-gated channels.}, url = {http://dx.doi.org/10.1016/j.neures.2005.10.009}, year = {2005} }

TY - JOUR ID - citeulike:409361 L3 - citeulike-article-id:409361 N2 - Neurons become photosensitive by genetically introducing one of green algae-derived protein, channelrhodopsin-2 (ChR2). Here, we quantitatively investigated the rapidness of the light-gated current of ChR2 expressed in PC12 cells using blue light-emitting diode (LED) light. The light-gated current consists of two components, inactivating and non-inactivating. The magnitude of inactivating component was almost linearly related to the light intensity. The non-inactivating component showed a tendency to saturate at high illumination. Both the activation and inactivation rates of the light-gated current were linearly dependent on the light intensity. However, the activation rate (turning-on rate) is about 10-fold faster than the inactivation rate. Although the turning-off time constant was little dependent on the light intensity, that at the end of 1s light pulse was about two-fold larger than that at 20ms. Neurons are also made photosensitive by the expression of ChR2 in the living animal. Since both the turning-on and turning-off time constants of light-gated current was smaller than the membrane time constant of neurons, the LED light illumination of the photosensitive neurons was enough to evoke action potentials in a pulse-to-pulse manner in an acute slice of hippocampus. JF - Neurosci Res SN - 0168-0102 AD - Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan. TI - Kinetic evaluation of photosensitivity in genetically engineered neurons expressing green algae light-gated channels. AU - Ishizuka, Toru AU - Kakuda, Masaaki AU - Araki, Rikita AU - Yawo, Hiromu PY - 2005/11/16/ UR - http://dx.doi.org/10.1016/j.neures.2005.10.009 ER -