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Nature reviews. Drug discovery, Vol. 12, No. 1. (31 December 2012), pp. 24-24, doi:10.1038/nrd3933 Key: citeulike:11862443
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The endoribonuclease Dicer heterogeneously cleaves short hairpin RNAs (shRNAs) into biologically active small interfering RNAs that have differing efficacies and off-target effects. This study found that promiscuous non-canonical cleavages by Dicer were prevented when the cleavage site was two nucleotides away from a bulge or
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