Interleukin-1beta, but not interleukin-6, enhances renal and systemic endothelin production in vivo Export

@article{citeulike:3200429, abstract = {The inflammatory cytokines IL-1{beta} and IL-6 have been shown to stimulate production of endothelin-1 (ET-1) by several cell types in vitro, but their effects on renal ET-1 production in vivo are not known. To test whether IL-1{beta} and IL-6 stimulate renal ET-1 production and release in vivo, urine was collected from male C57BL/6 mice over 24-h periods at baseline and on days 7 and 14 of a 14-day subcutaneous infusion of IL-1{beta} (10 ng/h), IL-6 (16 ng/h), or vehicle. By day 14, plasma ET-1 was significantly increased by IL-1{beta} infusion (1.7 {+/-} 0.1 vs. 0.8 {+/-} 0.1 pg/ml for vehicle, P < 0.001). Compared with vehicle infusion, IL-1{beta} infusion induced significant increases in urinary ET-1 excretion rate and urine flow but did not affect conscious mean arterial pressure (telemetry). IL-1{beta} infusion significantly increased renal cortical and medullary IL-1{beta} content (ELISA) and prepro-ET-1 mRNA expression (quantitative real-time PCR). In contrast, 14 days of IL-6 infusion had no significant effect on plasma ET-1 or urinary ET-1 excretion rate. To determine whether IL-1{beta} stimulates ET-1 release via activation of NF-{kappa}B, inner medullary collecting duct (IMCD-3) cells were incubated for 24 h with IL-1{beta}, and ET-1 release and NF-{kappa}B activation were measured (ELISA). IL-1{beta} activated NF-{kappa}B and increased ET-1 release in a concentration-dependent manner. The effect of IL-1{beta} on ET-1 release could be partially inhibited by pretreatment of IMCD-3 cells with an inhibitor of NF-{kappa}B activation (BAY 11-7082). These results indicate that IL-1{beta} stimulates renal and systemic ET-1 production in vivo, providing further evidence that ET-1 participates in inflammatory responses. 10.1152/ajprenal.00095.2008}, author = {Boesen, Erika I. and Sasser, Jennifer M. and Saleh, Mohamed A. and Potter, William A. and Woods, Mandy and Warner, Timothy D. and Pollock, Jennifer S. and Pollock, David M.}, citeulike-article-id = {3200429}, citeulike-linkout-0 = {http://dx.doi.org/10.1152/ajprenal.00095.2008}, citeulike-linkout-1 = {http://ajprenal.physiology.org/cgi/content/abstract/295/2/F446}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18524861}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18524861}, day = {1}, doi = {10.1152/ajprenal.00095.2008}, journal = {Am J Physiol Renal Physiol}, keywords = {et, il1, il6, kidney}, month = {August}, number = {2}, pages = {F446--453}, posted-at = {2008-09-07 10:11:33}, priority = {2}, title = {Interleukin-1{beta}, but not interleukin-6, enhances renal and systemic endothelin production in vivo}, url = {http://dx.doi.org/10.1152/ajprenal.00095.2008}, volume = {295}, year = {2008} }

TY - JOUR ID - citeulike:3200429 L3 - citeulike-article-id:3200429 N2 - The inflammatory cytokines IL-1{beta} and IL-6 have been shown to stimulate production of endothelin-1 (ET-1) by several cell types in vitro, but their effects on renal ET-1 production in vivo are not known. To test whether IL-1{beta} and IL-6 stimulate renal ET-1 production and release in vivo, urine was collected from male C57BL/6 mice over 24-h periods at baseline and on days 7 and 14 of a 14-day subcutaneous infusion of IL-1{beta} (10 ng/h), IL-6 (16 ng/h), or vehicle. By day 14, plasma ET-1 was significantly increased by IL-1{beta} infusion (1.7 {+/-} 0.1 vs. 0.8 {+/-} 0.1 pg/ml for vehicle, P < 0.001). Compared with vehicle infusion, IL-1{beta} infusion induced significant increases in urinary ET-1 excretion rate and urine flow but did not affect conscious mean arterial pressure (telemetry). IL-1{beta} infusion significantly increased renal cortical and medullary IL-1{beta} content (ELISA) and prepro-ET-1 mRNA expression (quantitative real-time PCR). In contrast, 14 days of IL-6 infusion had no significant effect on plasma ET-1 or urinary ET-1 excretion rate. To determine whether IL-1{beta} stimulates ET-1 release via activation of NF-{kappa}B, inner medullary collecting duct (IMCD-3) cells were incubated for 24 h with IL-1{beta}, and ET-1 release and NF-{kappa}B activation were measured (ELISA). IL-1{beta} activated NF-{kappa}B and increased ET-1 release in a concentration-dependent manner. The effect of IL-1{beta} on ET-1 release could be partially inhibited by pretreatment of IMCD-3 cells with an inhibitor of NF-{kappa}B activation (BAY 11-7082). These results indicate that IL-1{beta} stimulates renal and systemic ET-1 production in vivo, providing further evidence that ET-1 participates in inflammatory responses. 10.1152/ajprenal.00095.2008 IS - 2 JF - Am J Physiol Renal Physiol EP - 453 TI - Interleukin-1{beta}, but not interleukin-6, enhances renal and systemic endothelin production in vivo VL - 295 SP - F446 KW - et KW - il1 KW - il6 KW - kidney AU - Boesen, Erika AU - Sasser, Jennifer AU - Saleh, Mohamed AU - Potter, William AU - Woods, Mandy AU - Warner, Timothy AU - Pollock, Jennifer AU - Pollock, David PY - 2008/08/01/ UR - http://dx.doi.org/10.1152/ajprenal.00095.2008 ER -