High-Resolution Imaging of Redox Signaling in Live Cells Through an Oxidation-Sensitive Yellow Fluorescent Protein Export

@article{citeulike:3473763, abstract = {We present the application of a redox-sensitive mutant of the yellow fluorescent protein (rxYFP) to image, with elevated sensitivity and high temporal and spatial resolution, oxidative responses of eukaryotic cells to pathophysiological stimuli. The method presented, based on the ratiometric quantitation of the distribution of fluorescence by confocal microscopy, allows us to draw real-time "redox maps" of adherent cells and to score subtle changes in the intracellular redox state, such as those induced by overexpression of redox-active proteins. This strategy for in vivo imaging of redox signaling circumvents many of the technical limitations currently encountered in the study of complex redox-based phenomena and promises to contribute substantially to this expanding area of signal transduction. 10.1126/scisignal.143pl3}, author = {Maulucci, Giuseppe and Labate, Valentina and Mele, Marina and Panieri, Emiliano and Arcovito, Giuseppe and Galeotti, Tommaso and Ostergaard, Henrik and Winther, Jakob R. and De Spirito, Marco and Pani, Giovambattista}, citeulike-article-id = {3473763}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/scisignal.143pl3}, citeulike-linkout-1 = {http://stke.sciencemag.org/cgi/content/abstract/1/43/pl3}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18957692}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18957692}, day = {28}, doi = {10.1126/scisignal.143pl3}, journal = {Sci. Signal.}, keywords = {gfp, imaging, ros}, month = {October}, number = {43}, pages = {pl3+}, posted-at = {2008-11-25 01:46:16}, priority = {2}, title = {High-Resolution Imaging of Redox Signaling in Live Cells Through an Oxidation-Sensitive Yellow Fluorescent Protein}, url = {http://dx.doi.org/10.1126/scisignal.143pl3}, volume = {1}, year = {2008} }

TY - JOUR ID - citeulike:3473763 L3 - citeulike-article-id:3473763 N2 - We present the application of a redox-sensitive mutant of the yellow fluorescent protein (rxYFP) to image, with elevated sensitivity and high temporal and spatial resolution, oxidative responses of eukaryotic cells to pathophysiological stimuli. The method presented, based on the ratiometric quantitation of the distribution of fluorescence by confocal microscopy, allows us to draw real-time "redox maps" of adherent cells and to score subtle changes in the intracellular redox state, such as those induced by overexpression of redox-active proteins. This strategy for in vivo imaging of redox signaling circumvents many of the technical limitations currently encountered in the study of complex redox-based phenomena and promises to contribute substantially to this expanding area of signal transduction. 10.1126/scisignal.143pl3 IS - 43 JF - Sci. Signal. TI - High-Resolution Imaging of Redox Signaling in Live Cells Through an Oxidation-Sensitive Yellow Fluorescent Protein VL - 1 SP - pl3 KW - gfp KW - imaging KW - ros AU - Maulucci, Giuseppe AU - Labate, Valentina AU - Mele, Marina AU - Panieri, Emiliano AU - Arcovito, Giuseppe AU - Galeotti, Tommaso AU - Ostergaard, Henrik AU - Winther, Jakob AU - De Spirito, Marco AU - Pani, Giovambattista PY - 2008/10/28/ UR - http://dx.doi.org/10.1126/scisignal.143pl3 ER -