Analysis of urinary 8-oxo-7,8-dihydro-purine-2'-deoxyribonucleosides by LC-MS/MS and improved ELISA. Export

@article{citeulike:3739504, abstract = {Non-invasive monitoring of oxidative stress is highly desirable. Urinary 7,8-8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a biologically relevant and convenient analytical target. However, immunoassays can over-estimate levels of urinary 8-oxodG. Measurement of more than one DNA oxidation product in urine would be advantageous in terms of mechanistic information. Urines samples were analysed for 8-oxodG by solid-phase extraction/LC-MS/MS and ELISA. The solid-phase extraction/LC-MS/MS assay was also applied to the analysis of urinary 7,8-dihydro-8-oxo-2'-deoxyadenosine (8-oxodA). Concurring with previous reports, urinary 8-oxodG measured by ELISA was significantly higher than levels measured by LC-MS/MS. However, apparent improvement in the specificity of the commercially available Japanese Institute for the Control of Ageing (JaICA) ELISA brought mean LC-MS/MS and ELISA measurements of urinary 8-oxodG into agreement. Urinary 8-oxodA was undetectable in all urines, despite efficient recovery by solid phase extraction. Exploitation of the advantages of ELISA may be enhanced by a simple modification to the assay procedure, although chromatographic techniques still remain the 'gold standard' techniques for analysis of urinary 8-oxodG. Urinary 8-oxodA is either not present or below the limit of detection of the instrumentation.}, author = {Evans, M. D. and Singh, R. and Mistry, V. and Sandhu, K. and Farmer, P. B. and Cooke, M. S.}, citeulike-article-id = {3739504}, citeulike-linkout-0 = {http://dx.doi.org/10.1080/10715760802506323}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18985483}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18985483}, doi = {10.1080/10715760802506323}, issn = {1029-2470}, journal = {Free radical research}, keywords = {8ohdg, ms, ros, urine}, month = {October}, number = {10}, pages = {831--840}, posted-at = {2008-12-03 09:49:31}, priority = {2}, title = {Analysis of urinary 8-oxo-7,8-dihydro-purine-2'-deoxyribonucleosides by LC-MS/MS and improved ELISA.}, url = {http://dx.doi.org/10.1080/10715760802506323}, volume = {42}, year = {2008} }

TY - JOUR ID - citeulike:3739504 L3 - citeulike-article-id:3739504 N2 - Non-invasive monitoring of oxidative stress is highly desirable. Urinary 7,8-8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a biologically relevant and convenient analytical target. However, immunoassays can over-estimate levels of urinary 8-oxodG. Measurement of more than one DNA oxidation product in urine would be advantageous in terms of mechanistic information. Urines samples were analysed for 8-oxodG by solid-phase extraction/LC-MS/MS and ELISA. The solid-phase extraction/LC-MS/MS assay was also applied to the analysis of urinary 7,8-dihydro-8-oxo-2'-deoxyadenosine (8-oxodA). Concurring with previous reports, urinary 8-oxodG measured by ELISA was significantly higher than levels measured by LC-MS/MS. However, apparent improvement in the specificity of the commercially available Japanese Institute for the Control of Ageing (JaICA) ELISA brought mean LC-MS/MS and ELISA measurements of urinary 8-oxodG into agreement. Urinary 8-oxodA was undetectable in all urines, despite efficient recovery by solid phase extraction. Exploitation of the advantages of ELISA may be enhanced by a simple modification to the assay procedure, although chromatographic techniques still remain the 'gold standard' techniques for analysis of urinary 8-oxodG. Urinary 8-oxodA is either not present or below the limit of detection of the instrumentation. IS - 10 JF - Free radical research SN - 1029-2470 EP - 840 TI - Analysis of urinary 8-oxo-7,8-dihydro-purine-2'-deoxyribonucleosides by LC-MS/MS and improved ELISA. VL - 42 SP - 831 KW - 8ohdg KW - ms KW - ros KW - urine AU - Evans, MD AU - Singh, R AU - Mistry, V AU - Sandhu, K AU - Farmer, PB AU - Cooke, MS PY - 2008/10// UR - http://dx.doi.org/10.1080/10715760802506323 ER -