Plasma advanced glycation endproduct, methylglyoxal-derived hydroimidazolone is elevated in young, complication-free patients with Type 1 diabetes. Export

@article{citeulike:3993125, abstract = {OBJECTIVES: Elevated advanced glycation endproducts (AGEs) are implicated in diabetic complications. Methylglyoxal-derived hydroimidazolone (MG-H) is one of the most abundant AGEs in vivo. Our objective was to develop a time-saving, specific method to measure free MG-H in plasma and determine its levels in complication-free young individuals with Type 1 diabetes (T1DM). The relationship of plasma free MG-H to hemoglobin A1C (A1C) and plasma methylglyoxal levels was also determined. DESIGN AND METHODS: A solid phase extraction and liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed, and free plasma MG-H levels were measured in 40 T1DM patients (DM group), aged 6-21 years, and 11 non-diabetics (ND group), 6-22 years. Methylglyoxal was measured using LC-MS/MS and A1C by a Tosoh G7 high-performance liquid chromatograph. RESULTS: Our method showed high recovery, sensitivity and short run-time. Plasma free MG-H (nmol/L) was higher (p<0.001) in the DM group (1318+/-569; mean+/-standard deviation) as compared to the ND group (583+/-419). Within the DM group, plasma free MG-H did not correlate with plasma methylglyoxal or A1C. CONCLUSIONS: Our LC-MS/MS method to measure free MG-H in plasma may be useful for future clinical application. The increased levels of free MG-H observed in individuals with TIDM are not merely the result of short term changes in glucose or methylglyoxal, but may reflect long-term alterations to tissue proteins.}, author = {Han, Yingchun and Randell, Edward and Vasdev, Sudesh and Gill, Vicki and Curran, Matthew and Newhook, Leigh Anne A. and Grant, Marie and Hagerty, Donna and Schneider, Celine}, citeulike-article-id = {3993125}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.clinbiochem.2008.12.016}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0009912008006413}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19154730}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19154730}, day = {3}, doi = {10.1016/j.clinbiochem.2008.12.016}, issn = {1873-2933}, journal = {Clinical biochemistry}, keywords = {age, blood, dm, mgo}, month = {January}, posted-at = {2009-02-02 04:30:08}, priority = {2}, title = {Plasma advanced glycation endproduct, methylglyoxal-derived hydroimidazolone is elevated in young, complication-free patients with Type 1 diabetes.}, url = {http://dx.doi.org/10.1016/j.clinbiochem.2008.12.016}, year = {2009} }

TY - JOUR ID - citeulike:3993125 L3 - citeulike-article-id:3993125 N2 - OBJECTIVES: Elevated advanced glycation endproducts (AGEs) are implicated in diabetic complications. Methylglyoxal-derived hydroimidazolone (MG-H) is one of the most abundant AGEs in vivo. Our objective was to develop a time-saving, specific method to measure free MG-H in plasma and determine its levels in complication-free young individuals with Type 1 diabetes (T1DM). The relationship of plasma free MG-H to hemoglobin A1C (A1C) and plasma methylglyoxal levels was also determined. DESIGN AND METHODS: A solid phase extraction and liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed, and free plasma MG-H levels were measured in 40 T1DM patients (DM group), aged 6-21 years, and 11 non-diabetics (ND group), 6-22 years. Methylglyoxal was measured using LC-MS/MS and A1C by a Tosoh G7 high-performance liquid chromatograph. RESULTS: Our method showed high recovery, sensitivity and short run-time. Plasma free MG-H (nmol/L) was higher (p<0.001) in the DM group (1318+/-569; mean+/-standard deviation) as compared to the ND group (583+/-419). Within the DM group, plasma free MG-H did not correlate with plasma methylglyoxal or A1C. CONCLUSIONS: Our LC-MS/MS method to measure free MG-H in plasma may be useful for future clinical application. The increased levels of free MG-H observed in individuals with TIDM are not merely the result of short term changes in glucose or methylglyoxal, but may reflect long-term alterations to tissue proteins. JF - Clinical biochemistry SN - 1873-2933 TI - Plasma advanced glycation endproduct, methylglyoxal-derived hydroimidazolone is elevated in young, complication-free patients with Type 1 diabetes. KW - age KW - blood KW - dm KW - mgo AU - Han, Yingchun AU - Randell, Edward AU - Vasdev, Sudesh AU - Gill, Vicki AU - Curran, Matthew AU - Newhook, Leigh Anne AU - Grant, Marie AU - Hagerty, Donna AU - Schneider, Celine PY - 2009/01/03/ UR - http://dx.doi.org/10.1016/j.clinbiochem.2008.12.016 ER -