Alternative method for measurement of albumin/creatinine ratio using spectrophotometric sequential injection analysis. Export

@article{citeulike:4106026, abstract = {A simple, automatic and practical system for successive determination of albumin and creatinine has been developed by combining sequential injection analysis (SIA) and highly sensitive dye-binding assays. Albumin detection was based on the increase in the absorbance due to complex formation between albumin and eosin Y in acidic media. The absorbance of the complex was monitored at 547 nm. For the creatinine assay, the concentration of creatinine was measured by reaction with alkaline picrate to form a colored product which absorbs at 500 nm. The influences of experimental variables such as effects of pH, reagent concentration, standard/sample volume and interferences were investigated. Under optimal conditions, the automated method showed linearity up to 20 mg L(-1) for albumin and 100 mg L(-1) for creatinine. The 3 sigma detection limits were 0.6 and 3.5 mg L(-1) for albumin and creatinine, respectively, and the relative standard deviations (n=10) were 2.49\% for 20 mg L(-1) albumin, and 3.14\% for 20 mg L(-1) creatinine. Application of the proposed method to the direct analysis of urinary samples yielded results which agreed with those obtained from the Bradford protein assay and a creatinine enzymatic assay according to a paired t-test. The results obtained should be a step towards developing a fully automated and reliable analytical system for clinical research, which requires direct determination of albumin and creatinine and/or its ratios.}, author = {Siangproh, Weena and Teshima, Norio and Sakai, Tadao and Katoh, Shuji and Chailapakul, Orawon}, citeulike-article-id = {4106026}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.talanta.2008.12.068}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0039914009000617}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19615518}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19615518}, day = {15}, doi = {10.1016/j.talanta.2008.12.068}, issn = {1873-3573}, journal = {Talanta}, keywords = {hplc, uae}, month = {September}, number = {4}, pages = {1111--1117}, posted-at = {2009-02-26 08:38:40}, priority = {2}, title = {Alternative method for measurement of albumin/creatinine ratio using spectrophotometric sequential injection analysis.}, url = {http://dx.doi.org/10.1016/j.talanta.2008.12.068}, volume = {79}, year = {2009} }

TY - JOUR ID - citeulike:4106026 L3 - citeulike-article-id:4106026 N2 - A simple, automatic and practical system for successive determination of albumin and creatinine has been developed by combining sequential injection analysis (SIA) and highly sensitive dye-binding assays. Albumin detection was based on the increase in the absorbance due to complex formation between albumin and eosin Y in acidic media. The absorbance of the complex was monitored at 547 nm. For the creatinine assay, the concentration of creatinine was measured by reaction with alkaline picrate to form a colored product which absorbs at 500 nm. The influences of experimental variables such as effects of pH, reagent concentration, standard/sample volume and interferences were investigated. Under optimal conditions, the automated method showed linearity up to 20 mg L(-1) for albumin and 100 mg L(-1) for creatinine. The 3 sigma detection limits were 0.6 and 3.5 mg L(-1) for albumin and creatinine, respectively, and the relative standard deviations (n=10) were 2.49% for 20 mg L(-1) albumin, and 3.14% for 20 mg L(-1) creatinine. Application of the proposed method to the direct analysis of urinary samples yielded results which agreed with those obtained from the Bradford protein assay and a creatinine enzymatic assay according to a paired t-test. The results obtained should be a step towards developing a fully automated and reliable analytical system for clinical research, which requires direct determination of albumin and creatinine and/or its ratios. IS - 4 JF - Talanta SN - 1873-3573 EP - 1117 TI - Alternative method for measurement of albumin/creatinine ratio using spectrophotometric sequential injection analysis. VL - 79 SP - 1111 KW - hplc KW - uae AU - Siangproh, Weena AU - Teshima, Norio AU - Sakai, Tadao AU - Katoh, Shuji AU - Chailapakul, Orawon PY - 2009/09/15/ UR - http://dx.doi.org/10.1016/j.talanta.2008.12.068 ER -