Evidence of O-linked N-acetylglucosamine in diabetic nephropathy. Export

@article{citeulike:4275587, abstract = {AIMS: There is increasing evidence that O-linked N-acetylglucosamine (O-GlcNAc) plays an important role in cell signaling pathways. It has also been reported that increases in O-GlcNAc contribute to the development of diabetes and diabetic complications; however, little is known about O-GlcNAc levels in diabetic nephropathy (DNP). Therefore the goal of this study was to determine whether O-GlcNAc could be detected in human kidney biopsy specimens, and if so to examine whether O-GlcNAc levels were increased in the kidneys of patients with DNP compared to the non-diabetic individuals. MAIN METHODS: Kidney biopsy specimens were obtained from type-2 diabetic patients (n=6) and patients diagnosed with thin basement membrane nephropathy (n=7) were used as non-diabetic controls. O-GlcNAc levels were assessed by immunohistochemistry using the anti-O-GlcNAc antibody CTD110.6. KEY FINDINGS: We show that O-GlcNAc modification of proteins can be detected in the human kidney biopsy specimens. Furthermore, in diabetic patients, we found significantly increased numbers of O-GlcNAc positive cells in the glomeruli and significantly elevated staining in the tubuli (both in the nucleus and in the cytosol). In addition we also observed an intense, granular O-GlcNAc staining specifically in diabetic tubuli. SIGNIFICANCE: In light of the increase in O-GlcNAc staining in the diabetic patients, we propose that increased O-GlcNAc levels might contribute to the development of diabetic nephropathy.}, author = {Degrell, P\'{e}ter and Cseh, Judit and Moh\'{a}s, M\'{a}rton and Moln\'{a}r, Gergo A. and Pajor, L\'{a}szl\'{o} and Chatham, John C. and F\"{u}l\"{o}p, Norbert and Wittmann, Istv\'{a}n}, citeulike-article-id = {4275587}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.lfs.2009.01.007}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0024320509000368}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19302818}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19302818}, day = {27}, doi = {10.1016/j.lfs.2009.01.007}, issn = {1879-0631}, journal = {Life sciences}, keywords = {dn, oglcnac}, month = {March}, number = {13-14}, pages = {389--393}, posted-at = {2009-04-05 15:07:43}, priority = {2}, title = {Evidence of O-linked N-acetylglucosamine in diabetic nephropathy.}, url = {http://dx.doi.org/10.1016/j.lfs.2009.01.007}, volume = {84}, year = {2009} }

TY - JOUR ID - citeulike:4275587 L3 - citeulike-article-id:4275587 N2 - AIMS: There is increasing evidence that O-linked N-acetylglucosamine (O-GlcNAc) plays an important role in cell signaling pathways. It has also been reported that increases in O-GlcNAc contribute to the development of diabetes and diabetic complications; however, little is known about O-GlcNAc levels in diabetic nephropathy (DNP). Therefore the goal of this study was to determine whether O-GlcNAc could be detected in human kidney biopsy specimens, and if so to examine whether O-GlcNAc levels were increased in the kidneys of patients with DNP compared to the non-diabetic individuals. MAIN METHODS: Kidney biopsy specimens were obtained from type-2 diabetic patients (n=6) and patients diagnosed with thin basement membrane nephropathy (n=7) were used as non-diabetic controls. O-GlcNAc levels were assessed by immunohistochemistry using the anti-O-GlcNAc antibody CTD110.6. KEY FINDINGS: We show that O-GlcNAc modification of proteins can be detected in the human kidney biopsy specimens. Furthermore, in diabetic patients, we found significantly increased numbers of O-GlcNAc positive cells in the glomeruli and significantly elevated staining in the tubuli (both in the nucleus and in the cytosol). In addition we also observed an intense, granular O-GlcNAc staining specifically in diabetic tubuli. SIGNIFICANCE: In light of the increase in O-GlcNAc staining in the diabetic patients, we propose that increased O-GlcNAc levels might contribute to the development of diabetic nephropathy. IS - 13-14 JF - Life sciences SN - 1879-0631 EP - 393 TI - Evidence of O-linked N-acetylglucosamine in diabetic nephropathy. VL - 84 SP - 389 KW - dn KW - oglcnac AU - Degrell, Péter AU - Cseh, Judit AU - Mohás, Márton AU - Molnár, Gergo AU - Pajor, László AU - Chatham, John AU - Fülöp, Norbert AU - Wittmann, István PY - 2009/03/27/ UR - http://dx.doi.org/10.1016/j.lfs.2009.01.007 ER -