RNA interference using a plasmid construct expressing short-hairpin RNA. Export

@article{citeulike:4983932, abstract = {RNA interference (RNAi) is one of the most commonly used procedures for gene targeting in today's cutting edge technology and has great potential for use in clinical therapy. Using a plasmid construct that exogenously expresses short-hairpin RNAs (shRNAs) targeting a desired gene transcript not only helps to study the downstream effects of a gene product but also offers an alternative to viral vectors for gene therapy. Using a plasmid vector to knockdown a gene allows for long-term and permanent gene knockdown, without the need to generate knockout genotypes. Here, we detail the methodology for constructing a plasmid targeting the human telomerase reverse transcriptase (hTERT) gene through RNAi using the Ambion pSilencer system.}, author = {Lai, S. R. and Andrews, L. G. and Tollefsbol, T. O.}, citeulike-article-id = {4983932}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/978-1-60327-070-0_4}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18369815}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18369815}, doi = {10.1007/978-1-60327-070-0_4}, issn = {1064-3745}, journal = {Methods in molecular biology (Clifton, N.J.)}, keywords = {method, plasmid, rnai}, pages = {31--37}, posted-at = {2009-06-27 02:08:00}, priority = {2}, title = {RNA interference using a plasmid construct expressing short-hairpin RNA.}, url = {http://dx.doi.org/10.1007/978-1-60327-070-0_4}, volume = {405}, year = {2007} }

TY - JOUR ID - citeulike:4983932 L3 - citeulike-article-id:4983932 N2 - RNA interference (RNAi) is one of the most commonly used procedures for gene targeting in today's cutting edge technology and has great potential for use in clinical therapy. Using a plasmid construct that exogenously expresses short-hairpin RNAs (shRNAs) targeting a desired gene transcript not only helps to study the downstream effects of a gene product but also offers an alternative to viral vectors for gene therapy. Using a plasmid vector to knockdown a gene allows for long-term and permanent gene knockdown, without the need to generate knockout genotypes. Here, we detail the methodology for constructing a plasmid targeting the human telomerase reverse transcriptase (hTERT) gene through RNAi using the Ambion pSilencer system. JF - Methods in molecular biology (Clifton, N.J.) SN - 1064-3745 EP - 37 TI - RNA interference using a plasmid construct expressing short-hairpin RNA. VL - 405 SP - 31 KW - method KW - plasmid KW - rnai AU - Lai, SR AU - Andrews, LG AU - Tollefsbol, TO PY - 2007/// UR - http://dx.doi.org/10.1007/978-1-60327-070-0_4 ER -