Rapid, antibody-free detection of recombinant proteins on blots using enzyme fragment complementation. Export

@article{citeulike:5158962, abstract = {Alternative, antibody-free techniques to western analysis of protein blots can offer reduced assay times for routine analysis of expression of recombinant proteins. We have adapted the commercially available enzyme fragment complementation technology to provide a rapid protein detection method for protein blots based on significantly reducing the number of incubation and washing steps used in traditional approaches, and eliminating the requirement for antibodies. In this article, we highlight the use of this assay for measuring recombinant protein expressed in mammalian cells for a range of applications, including dot blot screening of large numbers of different cell samples, assessment of protein integrity through detection of degradation bands, and characterization of post-translational protein modifications such as glycosylation.}, author = {Charter, N. W. and Horecka, J. and Loh, C. Y. and Doan, A. and Wehrman, T. and Olson, K. R.}, citeulike-article-id = {5158962}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/978-1-59745-542-8_40}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19378077}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19378077}, doi = {10.1007/978-1-59745-542-8_40}, issn = {1064-3745}, journal = {Methods in molecular biology (Clifton, N.J.)}, keywords = {company, cost, eca, fca, wb}, pages = {395--405}, posted-at = {2009-07-15 14:34:37}, priority = {2}, title = {Rapid, antibody-free detection of recombinant proteins on blots using enzyme fragment complementation.}, url = {http://dx.doi.org/10.1007/978-1-59745-542-8_40}, volume = {536}, year = {2009} }

TY - JOUR ID - citeulike:5158962 L3 - citeulike-article-id:5158962 N2 - Alternative, antibody-free techniques to western analysis of protein blots can offer reduced assay times for routine analysis of expression of recombinant proteins. We have adapted the commercially available enzyme fragment complementation technology to provide a rapid protein detection method for protein blots based on significantly reducing the number of incubation and washing steps used in traditional approaches, and eliminating the requirement for antibodies. In this article, we highlight the use of this assay for measuring recombinant protein expressed in mammalian cells for a range of applications, including dot blot screening of large numbers of different cell samples, assessment of protein integrity through detection of degradation bands, and characterization of post-translational protein modifications such as glycosylation. JF - Methods in molecular biology (Clifton, N.J.) SN - 1064-3745 EP - 405 TI - Rapid, antibody-free detection of recombinant proteins on blots using enzyme fragment complementation. VL - 536 SP - 395 KW - company KW - cost KW - eca KW - fca KW - wb AU - Charter, NW AU - Horecka, J AU - Loh, CY AU - Doan, A AU - Wehrman, T AU - Olson, KR PY - 2009/// UR - http://dx.doi.org/10.1007/978-1-59745-542-8_40 ER -