Platelet-derived growth factor signaling through ephrin-b2 regulates hepatic vascular structure and function. Export

@article{citeulike:5289186, abstract = {BACKGROUND \& AIMS: Cirrhosis is associated with prominent changes in sinusoidal structure and function. Although the resident pericyte in liver, the hepatic stellate cell (HSC), is well characterized in the process of fibrogenesis, signaling pathways that regulate HSC vascular function are less developed. Because pericyte populations outside the liver are increasingly being recognized as a key cell type for angiogenesis and changes in vascular structure, in this study, we explore new HSC-signaling pathways that regulate sinusoidal structure and function. METHODS: Real-time video microscopy and quantitative software analysis of vascular tube formation were used to measure HSC angiogenesis in vitro. Platelet-derived growth factor (PDGF) and ephrin-signaling pathways were modulated using molecular and pharmacologic techniques. Complementary whole animal studies were performed to correlate in vitro findings with pericyte functions in vivo. RESULTS: We show that PDGF promotes a phenotype of HSC evidenced by enhanced HSC-driven vascular tube formation in vitro and enhanced HSC coverage of sinusoids in vivo. This angiogenic phenotype modulates specific pericyte vascular functions including permeability and pressure regulation. Furthermore, we identify a key role for ephrin-B2 as a downstream effector of PDGF signaling. CONCLUSIONS: These studies elucidate novel HSC-signaling pathways that regulate microvascular structure and function in liver.}, author = {Semela, D. and Das, A. and Langer, D. and Kang, N. and Leof, E. and Shah, V.}, citeulike-article-id = {5289186}, citeulike-linkout-0 = {http://dx.doi.org/10.1053/j.gastro.2008.04.010}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18570897}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18570897}, doi = {10.1053/j.gastro.2008.04.010}, issn = {1528-0012}, journal = {Gastroenterology}, keywords = {lc, pdgf, port-ht}, month = {August}, number = {2}, pages = {671--679}, posted-at = {2009-07-28 10:37:36}, priority = {2}, title = {Platelet-derived growth factor signaling through ephrin-b2 regulates hepatic vascular structure and function.}, url = {http://dx.doi.org/10.1053/j.gastro.2008.04.010}, volume = {135}, year = {2008} }

TY - JOUR ID - citeulike:5289186 L3 - citeulike-article-id:5289186 N2 - BACKGROUND & AIMS: Cirrhosis is associated with prominent changes in sinusoidal structure and function. Although the resident pericyte in liver, the hepatic stellate cell (HSC), is well characterized in the process of fibrogenesis, signaling pathways that regulate HSC vascular function are less developed. Because pericyte populations outside the liver are increasingly being recognized as a key cell type for angiogenesis and changes in vascular structure, in this study, we explore new HSC-signaling pathways that regulate sinusoidal structure and function. METHODS: Real-time video microscopy and quantitative software analysis of vascular tube formation were used to measure HSC angiogenesis in vitro. Platelet-derived growth factor (PDGF) and ephrin-signaling pathways were modulated using molecular and pharmacologic techniques. Complementary whole animal studies were performed to correlate in vitro findings with pericyte functions in vivo. RESULTS: We show that PDGF promotes a phenotype of HSC evidenced by enhanced HSC-driven vascular tube formation in vitro and enhanced HSC coverage of sinusoids in vivo. This angiogenic phenotype modulates specific pericyte vascular functions including permeability and pressure regulation. Furthermore, we identify a key role for ephrin-B2 as a downstream effector of PDGF signaling. CONCLUSIONS: These studies elucidate novel HSC-signaling pathways that regulate microvascular structure and function in liver. IS - 2 JF - Gastroenterology SN - 1528-0012 EP - 679 TI - Platelet-derived growth factor signaling through ephrin-b2 regulates hepatic vascular structure and function. VL - 135 SP - 671 KW - lc KW - pdgf KW - port-ht AU - Semela, D AU - Das, A AU - Langer, D AU - Kang, N AU - Leof, E AU - Shah, V PY - 2008/08// UR - http://dx.doi.org/10.1053/j.gastro.2008.04.010 ER -