(-)-Epigallocatechin 3-O-gallate (EGCG)-induced apoptosis in normal rat kidney interstitial fibroblast (NRK-49F) cells. Export

@article{citeulike:5626387, abstract = {(-)-Epigallocatechin 3-O-gallate (EGCG), a major catechin in green tea, suppresses renal failure in animals, and inhibits the growth of mesangial cells and opossum kidney proximal tubular cells. In addition, gallic acid, a structural constituent of this catechin, induces apoptosis in tumor cell lines. However, the effects of catechins on renal fibroblastic cells have not been investigated. In this experiment, the growth of normal rat kidney interstitial fibroblast (NRK-49F) cells was significantly inhibited by EGCG at concentrations higher than 6.25 microM, and almost completely inhibited at concentrations over 200 microM. The numbers of in situ end-labeled (ISEL) cells in cultures treated with EGCG at 6.25 to 200 microM increased dose-dependently. Furthermore, exposure to 6.25 to 50 microM EGCG for 24 hr led to a significant increase in caspase-3 activity compared to the control. These results suggest that EGCG induces apoptosis in NRK-49F cells.}, author = {Miyamoto, Yohei and Sano, Mariko and Haylor, John L. and El Nahas, A. Meguid}, citeulike-article-id = {5626387}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/18670168}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=18670168}, issn = {1880-3989}, journal = {The Journal of toxicological sciences}, keywords = {apoptosis, nrk49f, tea}, month = {August}, number = {3}, pages = {367--370}, posted-at = {2009-08-23 04:00:20}, priority = {2}, title = {(-)-Epigallocatechin 3-O-gallate (EGCG)-induced apoptosis in normal rat kidney interstitial fibroblast (NRK-49F) cells.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/18670168}, volume = {33}, year = {2008} }

TY - JOUR ID - citeulike:5626387 L3 - citeulike-article-id:5626387 N2 - (-)-Epigallocatechin 3-O-gallate (EGCG), a major catechin in green tea, suppresses renal failure in animals, and inhibits the growth of mesangial cells and opossum kidney proximal tubular cells. In addition, gallic acid, a structural constituent of this catechin, induces apoptosis in tumor cell lines. However, the effects of catechins on renal fibroblastic cells have not been investigated. In this experiment, the growth of normal rat kidney interstitial fibroblast (NRK-49F) cells was significantly inhibited by EGCG at concentrations higher than 6.25 microM, and almost completely inhibited at concentrations over 200 microM. The numbers of in situ end-labeled (ISEL) cells in cultures treated with EGCG at 6.25 to 200 microM increased dose-dependently. Furthermore, exposure to 6.25 to 50 microM EGCG for 24 hr led to a significant increase in caspase-3 activity compared to the control. These results suggest that EGCG induces apoptosis in NRK-49F cells. IS - 3 JF - The Journal of toxicological sciences SN - 1880-3989 EP - 370 TI - (-)-Epigallocatechin 3-O-gallate (EGCG)-induced apoptosis in normal rat kidney interstitial fibroblast (NRK-49F) cells. VL - 33 SP - 367 KW - apoptosis KW - nrk49f KW - tea AU - Miyamoto, Yohei AU - Sano, Mariko AU - Haylor, John AU - El Nahas, Meguid PY - 2008/08// UR - http://view.ncbi.nlm.nih.gov/pubmed/18670168 ER -