Stabilization of hepatocyte growth factor mRNA by hypoxia-inducible factor 1. Export

@article{citeulike:5834787, abstract = {Hypoxia regulates expression of hepatocyte growth factor (HGF) by increasing its transcription and by stabilizing its mRNA. Despite the pivotal role of hypoxia-inducible factor 1 (HIF-1) in transcriptional activation of hypoxia-responsive genes, it is not known whether HIF-1 mediates hypoxia-induced stabilization of HGF mRNA. We constructed adenoviral vectors expressing either the wild-type HIF-1alpha (Ad2/HIF-1alpha/FL), a constitutively stable hybrid form of HIF-1alpha (Ad2/HIF-1alpha/VP16), or no transgene (Ad2/CMVEV). In rat glioma (C6) cells, human glioma (U251) cells human cardiac, vascular smooth muscle, and endothelial cells, infection with Ad2/HIF-1alpha/VP16 or Ad2/HIF-1alpha/FL increased HGF expression at both the mRNA and protein levels. Under normoxic conditions, the half-life of HGF mRNA was 43 min in C6 and U251 cells. Hypoxia and Ad2/HIF-1alpha/VP16 increased the half-life of HGF mRNA to 3.2 and 2.8 h, respectively, while Ad2/CMVEV had no effect. These studies are the first to demonstrate that overexpression of HIF-1alpha increases HGF mRNA stability. Our results also suggest that stabilization of HGF mRNA by hypoxia is mediated, at least in part, by HIF-1.}, author = {Chu, Sheng-Hua H. and Feng, Dong-Fu F. and Ma, Yan-Bin B. and Zhu, Zhi-An A. and Zhang, Hong and Qiu, Jian-Hua H.}, citeulike-article-id = {5834787}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s11033-008-9406-1}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18979225}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18979225}, doi = {10.1007/s11033-008-9406-1}, issn = {1573-4978}, journal = {Molecular biology reports}, keywords = {hgf, hif1}, month = {September}, number = {7}, pages = {1967--1975}, posted-at = {2009-09-24 06:09:43}, priority = {2}, title = {Stabilization of hepatocyte growth factor mRNA by hypoxia-inducible factor 1.}, url = {http://dx.doi.org/10.1007/s11033-008-9406-1}, volume = {36}, year = {2009} }

TY - JOUR ID - citeulike:5834787 L3 - citeulike-article-id:5834787 N2 - Hypoxia regulates expression of hepatocyte growth factor (HGF) by increasing its transcription and by stabilizing its mRNA. Despite the pivotal role of hypoxia-inducible factor 1 (HIF-1) in transcriptional activation of hypoxia-responsive genes, it is not known whether HIF-1 mediates hypoxia-induced stabilization of HGF mRNA. We constructed adenoviral vectors expressing either the wild-type HIF-1alpha (Ad2/HIF-1alpha/FL), a constitutively stable hybrid form of HIF-1alpha (Ad2/HIF-1alpha/VP16), or no transgene (Ad2/CMVEV). In rat glioma (C6) cells, human glioma (U251) cells human cardiac, vascular smooth muscle, and endothelial cells, infection with Ad2/HIF-1alpha/VP16 or Ad2/HIF-1alpha/FL increased HGF expression at both the mRNA and protein levels. Under normoxic conditions, the half-life of HGF mRNA was 43 min in C6 and U251 cells. Hypoxia and Ad2/HIF-1alpha/VP16 increased the half-life of HGF mRNA to 3.2 and 2.8 h, respectively, while Ad2/CMVEV had no effect. These studies are the first to demonstrate that overexpression of HIF-1alpha increases HGF mRNA stability. Our results also suggest that stabilization of HGF mRNA by hypoxia is mediated, at least in part, by HIF-1. IS - 7 JF - Molecular biology reports SN - 1573-4978 EP - 1975 TI - Stabilization of hepatocyte growth factor mRNA by hypoxia-inducible factor 1. VL - 36 SP - 1967 KW - hgf KW - hif1 AU - Chu, Sheng-Hua AU - Feng, Dong-Fu AU - Ma, Yan-Bin AU - Zhu, Zhi-An AU - Zhang, Hong AU - Qiu, Jian-Hua PY - 2009/09// UR - http://dx.doi.org/10.1007/s11033-008-9406-1 ER -