Simultaneous Determination Of Angiotensin Ii And 1-7 By Capillary Zone Electrophoresis In Plasma And Urine From Hypertensive Rats Export

@article{citeulike:5948236, abstract = {We describe the procedure developed for the simultaneous detection and quantification of angiotensin II and angiotensin-(1-7), by capillary zone electrophoresis with UV detection by photodiode-array, at a wavelength of 200 nm, in the plasma and urine from hypertensive rats. Optimal separation was achieved with a 100 mM boric acid + 3 mM tartaric acid + 10 fM gold (III) chloride electrolyte solution at pH 9.80. The applied voltage was 30 kV and the capillary temperature was kept constant at 20 °C. The method was over the concentration range of 0.01-500 pmoles/mL. All determination coefficients were higher or equal to 0.9985. Limits of detection and quantification for angiotensin II were 0.0110 pmoles/mL (S/N=3) and 0.0195 pmoles/mL (S/N = 5), respectively. While for angiotensin-(1-7), the limits were 0.0112 pmoles/mL (S/N = 3) and 0.0193 pmoles/mL (S/N = 5), respectively. The present method offers a time-saving way to simultaneous determination of angiotensin II and angiotensin-(1-7), since it can be completed in 10 min, compared to other methodologies reported in the literature for capillary electrophoresis and liquid chromatography, which require more than 1 h for analysis of complex matrices, such as plasma and urine. The procedure is illustrated by experiments that quantify simultaneously angiotensin II and angiotensin-(1-7) in plasma and urine from hypertensive and normotensive rats, with and without antihypertensive treatment. The levels of angiotensin II and angiotensin-(1-7) detected in the experimental model, resulted in a recovery of 99.00 to 106.01\% and a reproducibility of less than 10\%. The proposed analytical method is a use full tool for the simultaneous detection of angiotensin II and angiotensin-(1-7) implicated in vascular remodeling in pathologies such as hypertension.}, author = {Tenorio-L\'{o}pez, Ferm\'{\i}n A. and Zarco-Olvera, Gabriela and S\'{a}nchez-Mendoza, Alicia and Rosas-Peralta, Mart\'{\i}n and Pastel\'{\i}n-Hern\'{a}ndez, Gustavo and del Valle-Mondrag\'{o}n, Leonardo}, citeulike-article-id = {5948236}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.talanta.2009.10.010}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0039914009007991}, day = {13}, doi = {10.1016/j.talanta.2009.10.010}, issn = {00399140}, journal = {Talanta}, keywords = {ace2, aii, method, rat}, month = {October}, posted-at = {2009-11-04 04:30:09}, priority = {2}, title = {Simultaneous Determination Of Angiotensin Ii And 1-7 By Capillary Zone Electrophoresis In Plasma And Urine From Hypertensive Rats}, url = {http://dx.doi.org/10.1016/j.talanta.2009.10.010}, year = {2009} }

TY - JOUR ID - citeulike:5948236 L3 - citeulike-article-id:5948236 N2 - We describe the procedure developed for the simultaneous detection and quantification of angiotensin II and angiotensin-(1-7), by capillary zone electrophoresis with UV detection by photodiode-array, at a wavelength of 200 nm, in the plasma and urine from hypertensive rats. Optimal separation was achieved with a 100 mM boric acid + 3 mM tartaric acid + 10 fM gold (III) chloride electrolyte solution at pH 9.80. The applied voltage was 30 kV and the capillary temperature was kept constant at 20 °C. The method was over the concentration range of 0.01-500 pmoles/mL. All determination coefficients were higher or equal to 0.9985. Limits of detection and quantification for angiotensin II were 0.0110 pmoles/mL (S/N=3) and 0.0195 pmoles/mL (S/N = 5), respectively. While for angiotensin-(1-7), the limits were 0.0112 pmoles/mL (S/N = 3) and 0.0193 pmoles/mL (S/N = 5), respectively. The present method offers a time-saving way to simultaneous determination of angiotensin II and angiotensin-(1-7), since it can be completed in 10 min, compared to other methodologies reported in the literature for capillary electrophoresis and liquid chromatography, which require more than 1 h for analysis of complex matrices, such as plasma and urine. The procedure is illustrated by experiments that quantify simultaneously angiotensin II and angiotensin-(1-7) in plasma and urine from hypertensive and normotensive rats, with and without antihypertensive treatment. The levels of angiotensin II and angiotensin-(1-7) detected in the experimental model, resulted in a recovery of 99.00 to 106.01% and a reproducibility of less than 10%. The proposed analytical method is a use full tool for the simultaneous detection of angiotensin II and angiotensin-(1-7) implicated in vascular remodeling in pathologies such as hypertension. JF - Talanta SN - 00399140 TI - Simultaneous Determination Of Angiotensin Ii And 1-7 By Capillary Zone Electrophoresis In Plasma And Urine From Hypertensive Rats KW - ace2 KW - aii KW - method KW - rat AU - Tenorio-López, Fermín AU - Zarco-Olvera, Gabriela AU - Sánchez-Mendoza, Alicia AU - Rosas-Peralta, Martín AU - Pastelín-Hernández, Gustavo AU - del Valle-Mondragón, Leonardo PY - 2009/10/13/ UR - http://dx.doi.org/10.1016/j.talanta.2009.10.010 ER -