Nucleic acid aptamers for biosensors and bio-analytical applications. Export

@article{citeulike:6072688, abstract = {Oligonucleotides were once considered only functional as molecules for the storage of genetic information. However, the discovery of RNAzymes, and later, DNAzymes, unravelled the innate potential of oligonucleotides in many other biological applications. In the last two decades, these applications have been further expanded through the introduction of Systematic Evolution of Ligands by EXponential enrichment (SELEX) which has generated, by repeated rounds of in vitro selection, a type of molecular probe termed aptamers. Aptamers are oligonucleic acid (or peptide) molecules that can bind to various molecular targets and are viewed as complements to antibodies. Aptamers have found applications in many areas, such as bio-technology, medicine, pharmacology, microbiology, and analytical chemistry, including chromatographic separation and biosensors. In this review, we focus on the use of aptamers in the development of biosensors. Coupled with their ability to bind a variety of targets, the robust nature of oligonucleotides, in terms of synthesis, storage, and wide range of temperature stability and chemical manipulation, makes them highly suitable for biosensor design and engineering. Among the many design strategies, we discuss three general paradigms that have appeared most frequently in the literature: structure-switching, enzyme-based, and aptazyme-based designs.}, author = {Sefah, Kwame and Phillips, Joseph A. and Xiong, Xiangling and Meng, Ling and Van Simaeys, Dimitri and Chen, Hui and Martin, Jennifer and Tan, Weihong}, citeulike-article-id = {6072688}, citeulike-linkout-0 = {http://dx.doi.org/10.1039/b905609m}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19684896}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19684896}, doi = {10.1039/b905609m}, issn = {1364-5528}, journal = {The Analyst}, keywords = {biosensor}, month = {September}, number = {9}, pages = {1765--1775}, posted-at = {2009-11-05 08:56:36}, priority = {2}, title = {Nucleic acid aptamers for biosensors and bio-analytical applications.}, url = {http://dx.doi.org/10.1039/b905609m}, volume = {134}, year = {2009} }

TY - JOUR ID - citeulike:6072688 L3 - citeulike-article-id:6072688 N2 - Oligonucleotides were once considered only functional as molecules for the storage of genetic information. However, the discovery of RNAzymes, and later, DNAzymes, unravelled the innate potential of oligonucleotides in many other biological applications. In the last two decades, these applications have been further expanded through the introduction of Systematic Evolution of Ligands by EXponential enrichment (SELEX) which has generated, by repeated rounds of in vitro selection, a type of molecular probe termed aptamers. Aptamers are oligonucleic acid (or peptide) molecules that can bind to various molecular targets and are viewed as complements to antibodies. Aptamers have found applications in many areas, such as bio-technology, medicine, pharmacology, microbiology, and analytical chemistry, including chromatographic separation and biosensors. In this review, we focus on the use of aptamers in the development of biosensors. Coupled with their ability to bind a variety of targets, the robust nature of oligonucleotides, in terms of synthesis, storage, and wide range of temperature stability and chemical manipulation, makes them highly suitable for biosensor design and engineering. Among the many design strategies, we discuss three general paradigms that have appeared most frequently in the literature: structure-switching, enzyme-based, and aptazyme-based designs. IS - 9 JF - The Analyst SN - 1364-5528 EP - 1775 TI - Nucleic acid aptamers for biosensors and bio-analytical applications. VL - 134 SP - 1765 KW - biosensor AU - Sefah, Kwame AU - Phillips, Joseph AU - Xiong, Xiangling AU - Meng, Ling AU - Van Simaeys, Dimitri AU - Chen, Hui AU - Martin, Jennifer AU - Tan, Weihong PY - 2009/09// UR - http://dx.doi.org/10.1039/b905609m ER -