Spatial and Mechanical Properties of Dilute DNA Monolayers on Gold Imaged by AFM

@article{citeulike:2385790, abstract = {Abstract: Spatially distributed DNA oligomer arrays on Au(111) surfaces were created by one-step co-assembly of mixed monolayers of alkanethiol-conjugated DNA and mercaptohexanol (MCH). Tapping-mode AFM was used to visualize the distribution of DNA molecules on the surface and to study the mechanical properties of individual molecules. The DNA coating density increased nonlinearly with increasing mole fraction of DNA oligomer to MCH in the coating solution. For imaging in air, where surfaces are coated with a thin layer of water, the interaction between the AFM tip and the different structures on the monolayer varies between attractive and repulsive depending on the tapping amplitude, set-point ratio, and tip shape. It was found that both duplex and single-stranded DNA molecules extend approximately vertically upward from the surface.}, address = {Institute of Chemical Physics, University of Latvia, Rainis Boulevard 19, LV-1586 Riga, Latvia, Physics and Engineering Physics, Chalmers University of Technology, SE-412 96 G\"{o}teborg, Sweden, and Department of Chemistry, Bedson Building, University of Newcastle, Newcastle upon Tyne NE1 7RU, U.K.}, author = {Erts, D. and Polyakov, B. and Olin, H. and Tuite, E. }, citeulike-article-id = {2385790}, doi = {10.1021/jp0261657}, journal = {J. Phys. Chem. B}, keywords = {hakanolinpublicationlist}, month = {April}, number = {15}, pages = {3591--3597}, posted-at = {2008-02-15 13:33:12}, priority = {2}, title = {Spatial and Mechanical Properties of Dilute DNA Monolayers on Gold Imaged by AFM}, url = {http://dx.doi.org/10.1021/jp0261657}, volume = {107}, year = {2003} }

TY - JOUR ID - citeulike:2385790 L3 - citeulike-article-id:2385790 TI - Spatial and Mechanical Properties of Dilute DNA Monolayers on Gold Imaged by AFM JF - J. Phys. Chem. B VL - 107 IS - 15 SP - 3591 EP - 3597 AD - Institute of Chemical Physics, University of Latvia, Rainis Boulevard 19, LV-1586 Riga, Latvia, Physics and Engineering Physics, Chalmers University of Technology, SE-412 96 Göteborg, Sweden, and Department of Chemistry, Bedson Building, University of Newcastle, Newcastle upon Tyne NE1 7RU, U.K. N2 - Abstract: Spatially distributed DNA oligomer arrays on Au(111) surfaces were created by one-step co-assembly of mixed monolayers of alkanethiol-conjugated DNA and mercaptohexanol (MCH). Tapping-mode AFM was used to visualize the distribution of DNA molecules on the surface and to study the mechanical properties of individual molecules. The DNA coating density increased nonlinearly with increasing mole fraction of DNA oligomer to MCH in the coating solution. For imaging in air, where surfaces are coated with a thin layer of water, the interaction between the AFM tip and the different structures on the monolayer varies between attractive and repulsive depending on the tapping amplitude, set-point ratio, and tip shape. It was found that both duplex and single-stranded DNA molecules extend approximately vertically upward from the surface. KW - hakanolinpublicationlist AU - Erts, D AU - Polyakov, B AU - Olin, H AU - Tuite, E PY - 2003/04/17/ UR - http://dx.doi.org/10.1021/jp0261657 ER -