A Molecular Determinant for the Establishment of Sister Chromatid Cohesion

@article{citeulike:3047387, abstract = {Chromosome segregation, transcriptional regulation, and repair of DNA double-strand breaks require the cohesin protein complex. Cohesin holds the replicated chromosomes (sister chromatids) together to mediate sister chromatid cohesion. The mechanism of how cohesion is established is unknown. We found that in budding yeast, the head domain of the Smc3p subunit of cohesin is acetylated by the Eco1p acetyltransferase at two evolutionarily conserved residues, promoting the chromatin-bound cohesin to tether sister chromatids. Smc3p acetylation is induced in S phase after the chromatin loading of cohesin and is suppressed in G1 and G2/M. Smc3 head acetylation and its cell cycle regulation provide important insights into the biology and mechanism of cohesion establishment. 10.1126/science.1157880}, author = {Unal, Elcin and Heidinger-Pauli, Jill M. and Kim, Woong and Guacci, Vincent and Onn, Itay and Gygi, Steven P. and Koshland, Douglas E.}, citeulike-article-id = {3047387}, doi = {10.1126/science.1157880}, journal = {Science}, keywords = {chromatin-interchromosmomal}, month = {July}, number = {5888}, pages = {566--569}, posted-at = {2008-07-28 05:47:47}, priority = {4}, title = {A Molecular Determinant for the Establishment of Sister Chromatid Cohesion}, url = {http://dx.doi.org/10.1126/science.1157880}, volume = {321}, year = {2008} }

TY - JOUR ID - citeulike:3047387 L3 - citeulike-article-id:3047387 N2 - Chromosome segregation, transcriptional regulation, and repair of DNA double-strand breaks require the cohesin protein complex. Cohesin holds the replicated chromosomes (sister chromatids) together to mediate sister chromatid cohesion. The mechanism of how cohesion is established is unknown. We found that in budding yeast, the head domain of the Smc3p subunit of cohesin is acetylated by the Eco1p acetyltransferase at two evolutionarily conserved residues, promoting the chromatin-bound cohesin to tether sister chromatids. Smc3p acetylation is induced in S phase after the chromatin loading of cohesin and is suppressed in G1 and G2/M. Smc3 head acetylation and its cell cycle regulation provide important insights into the biology and mechanism of cohesion establishment. 10.1126/science.1157880 IS - 5888 JF - Science EP - 569 TI - A Molecular Determinant for the Establishment of Sister Chromatid Cohesion VL - 321 SP - 566 KW - chromatin-interchromosmomal AU - Unal, Elcin AU - Heidinger-Pauli, Jill AU - Kim, Woong AU - Guacci, Vincent AU - Onn, Itay AU - Gygi, Steven AU - Koshland, Douglas PY - 2008/07/25/ UR - http://dx.doi.org/10.1126/science.1157880 ER -