<i>J. Cosmet. Sci., </i> <b>59</b>, 195&#x2013;202 (May/June 2008) The effects of placental extract on fibroblast proliferation Export

@article{citeulike:4500709, abstract = {Human placental extract is used in the treatment of skin wrinkles and wounds. To date, no studies have evaluated the effects of placental extract on dermal fibroblast proliferation. To investigate the effects of placental extract vs. ascorbic acid on fibroblast proliferation and transforming growth factor (TGF)-03B21 expression, cultured human fibroblasts were treated with placental extract (0\%, 0.08\%, 0.16\%, 0.32\%, and 0.64\%) or L-ascorbic acid-2-phosphate magnesium (0, 0.01, 0.1, 1.0, and 10 mmol L-1). Fibroblast proliferation was determined by MTT assay, and TGF-03B21 protein expression was analyzed by ELISA. The proliferation of fibroblasts increased significantly after treatment with placental extract at concentrations of 0.32\% and 0.64\% and with L-ascorbic acid-2-phosphate magnesium at concentrations of 1.0 and 10 mmol L-1. Placental extract demonstrated no significant effects on TGF-03B21 expression; however, TGF-03B21 expression significantly increased after treatment with ascorbic acid at concentrations of 1.0 and 10 mmol L-1. Placental extract and ascorbic acid had similar effects on fibroblast proliferation; however, placental extract did not significantly increase TGF-03B21 protein expression.}, address = {Department of Dermatology (H.-R.C., M.-H.L.) and East-West Medical Research Institute (J.-W.L.), College of Medicine, Kyunghee University and Anacli Dermatologic Clinic (J.-H.R.), Seoul, Korea.}, author = {Cho, H. R. and Ryou, J. H. and Lee, J. W. and Lee, M. H.}, citeulike-article-id = {4500709}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1468-2494.2009.00462_1.x}, citeulike-linkout-1 = {http://www.ingentaconnect.com/content/bsc/ics/2009/00000031/00000003/art00013}, citeulike-linkout-2 = {http://www3.interscience.wiley.com/cgi-bin/abstract/122380757/ABSTRACT}, doi = {10.1111/j.1468-2494.2009.00462_1.x}, issn = {0142-5463}, journal = {International Journal of Cosmetic Science}, keywords = {anti-aging, cosmetic, dermatology, molecular-biology, placenta-extract}, number = {3}, pages = {240}, posted-at = {2009-07-01 06:45:20}, priority = {2}, publisher = {Blackwell Publishing}, title = {<i>J. Cosmet. Sci., </i>\&nbsp;<b>59</b>, 195\&\#x2013;202 (May/June 2008) The effects of placental extract on fibroblast proliferation}, url = {http://dx.doi.org/10.1111/j.1468-2494.2009.00462_1.x}, volume = {31}, year = {2009} }

TY - JOUR ID - citeulike:4500709 L3 - citeulike-article-id:4500709 N2 - Human placental extract is used in the treatment of skin wrinkles and wounds. To date, no studies have evaluated the effects of placental extract on dermal fibroblast proliferation. To investigate the effects of placental extract vs. ascorbic acid on fibroblast proliferation and transforming growth factor (TGF)-03B21 expression, cultured human fibroblasts were treated with placental extract (0%, 0.08%, 0.16%, 0.32%, and 0.64%) or L-ascorbic acid-2-phosphate magnesium (0, 0.01, 0.1, 1.0, and 10 mmol L-1). Fibroblast proliferation was determined by MTT assay, and TGF-03B21 protein expression was analyzed by ELISA. The proliferation of fibroblasts increased significantly after treatment with placental extract at concentrations of 0.32% and 0.64% and with L-ascorbic acid-2-phosphate magnesium at concentrations of 1.0 and 10 mmol L-1. Placental extract demonstrated no significant effects on TGF-03B21 expression; however, TGF-03B21 expression significantly increased after treatment with ascorbic acid at concentrations of 1.0 and 10 mmol L-1. Placental extract and ascorbic acid had similar effects on fibroblast proliferation; however, placental extract did not significantly increase TGF-03B21 protein expression. IS - 3 JF - International Journal of Cosmetic Science SN - 0142-5463 AD - Department of Dermatology (H.-R.C., M.-H.L.) and East-West Medical Research Institute (J.-W.L.), College of Medicine, Kyunghee University and Anacli Dermatologic Clinic (J.-H.R.), Seoul, Korea. EP - 240 TI - <i>J. Cosmet. Sci., </i>&nbsp;<b>59</b>, 195&#x2013;202 (May/June 2008) The effects of placental extract on fibroblast proliferation PB - Blackwell Publishing VL - 31 SP - 240 KW - anti-aging KW - cosmetic KW - dermatology KW - molecular-biology KW - placenta-extract AU - Cho, HR AU - Ryou, JH AU - Lee, JW AU - Lee, MH PY - 2009/// UR - http://dx.doi.org/10.1111/j.1468-2494.2009.00462_1.x ER -