The role of dynamic conformational ensembles in biomolecular recognition Export

@article{citeulike:5972833, abstract = {Molecular recognition is central to all biological processes. For the past 50 years, Koshland's 'induced fit' hypothesis has been the textbook explanation for molecular recognition events. However, recent experimental evidence supports an alternative mechanism. 'Conformational selection' postulates that all protein conformations pre-exist, and the ligand selects the most favored conformation. Following binding the ensemble undergoes a population shift, redistributing the conformational states. Both conformational selection and induced fit appear to play roles. Following binding by a primary conformational selection event, optimization of side chain and backbone interactions is likely to proceed by an induced fit mechanism. Conformational selection has been observed for protein-ligand, protein-protein, protein-DNA, protein-RNA and RNA-ligand interactions. These data support a new molecular recognition paradigm for processes as diverse as signaling, catalysis, gene regulation and protein aggregation in disease, which has the potential to significantly impact our views and strategies in drug design, biomolecular engineering and molecular evolution.}, author = {Boehr, David D. and Nussinov, Ruth and Wright, Peter E.}, citeulike-article-id = {5972833}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nchembio.232}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nchembio.232}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19841628}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19841628}, day = {10}, doi = {10.1038/nchembio.232}, issn = {1552-4450}, journal = {Nature Chemical Biology}, keywords = {2009, flexibility, scissors}, month = {October}, number = {11}, pages = {789--796}, posted-at = {2009-11-03 12:19:15}, priority = {2}, publisher = {Nature Publishing Group}, title = {The role of dynamic conformational ensembles in biomolecular recognition}, url = {http://dx.doi.org/10.1038/nchembio.232}, volume = {5}, year = {2009} }

TY - JOUR ID - citeulike:5972833 L3 - citeulike-article-id:5972833 N2 - Molecular recognition is central to all biological processes. For the past 50 years, Koshland's 'induced fit' hypothesis has been the textbook explanation for molecular recognition events. However, recent experimental evidence supports an alternative mechanism. 'Conformational selection' postulates that all protein conformations pre-exist, and the ligand selects the most favored conformation. Following binding the ensemble undergoes a population shift, redistributing the conformational states. Both conformational selection and induced fit appear to play roles. Following binding by a primary conformational selection event, optimization of side chain and backbone interactions is likely to proceed by an induced fit mechanism. Conformational selection has been observed for protein-ligand, protein-protein, protein-DNA, protein-RNA and RNA-ligand interactions. These data support a new molecular recognition paradigm for processes as diverse as signaling, catalysis, gene regulation and protein aggregation in disease, which has the potential to significantly impact our views and strategies in drug design, biomolecular engineering and molecular evolution. IS - 11 JF - Nature Chemical Biology SN - 1552-4450 EP - 796 TI - The role of dynamic conformational ensembles in biomolecular recognition PB - Nature Publishing Group VL - 5 SP - 789 KW - 2009 KW - flexibility KW - scissors AU - Boehr, David AU - Nussinov, Ruth AU - Wright, Peter PY - 2009/10/10/ UR - http://dx.doi.org/10.1038/nchembio.232 ER -