In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state Export

@article{citeulike:1369604, abstract = {Nuclear transplantation can reprogramme a somatic genome back into an embryonic epigenetic state, and the reprogrammed nucleus can create a cloned animal or produce pluripotent embryonic stem cells. One potential use of the nuclear cloning approach is the derivation of 'customized' embryonic stem (ES) cells for patient-specific cell treatment, but technical and ethical considerations impede the therapeutic application of this technology. Reprogramming of fibroblasts to a pluripotent state can be induced in vitro through ectopic expression of the four transcription factors Oct4 (also called Oct3/4 or Pou5f1), Sox2, c-Myc and Klf4. Here we show that DNA methylation, gene expression and chromatin state of such induced reprogrammed stem cells are similar to those of ES cells. Notably, the cells—derived from mouse fibroblasts—can form viable chimaeras, can contribute to the germ line and can generate live late-term embryos when injected into tetraploid blastocysts. Our results show that the biological potency and epigenetic state of in-vitro-reprogrammed induced pluripotent stem cells are indistinguishable from those of ES cells.}, author = {Wernig, Marius and Meissner, Alexander and Foreman, Ruth and Brambrink, Tobias and Ku, Manching and Hochedlinger, Konrad and Bernstein, Bradley E. and Jaenisch, Rudolf}, citeulike-article-id = {1369604}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nature05944}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nature05944}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17554336}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17554336}, day = {06}, doi = {10.1038/nature05944}, issn = {0028-0836}, journal = {Nature}, keywords = {nsf}, month = {June}, number = {7151}, pages = {318--324}, posted-at = {2009-11-06 02:38:39}, priority = {2}, publisher = {Nature Publishing Group}, title = {In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state}, url = {http://dx.doi.org/10.1038/nature05944}, volume = {448}, year = {2007} }

TY - JOUR ID - citeulike:1369604 L3 - citeulike-article-id:1369604 N2 - Nuclear transplantation can reprogramme a somatic genome back into an embryonic epigenetic state, and the reprogrammed nucleus can create a cloned animal or produce pluripotent embryonic stem cells. One potential use of the nuclear cloning approach is the derivation of 'customized' embryonic stem (ES) cells for patient-specific cell treatment, but technical and ethical considerations impede the therapeutic application of this technology. Reprogramming of fibroblasts to a pluripotent state can be induced in vitro through ectopic expression of the four transcription factors Oct4 (also called Oct3/4 or Pou5f1), Sox2, c-Myc and Klf4. Here we show that DNA methylation, gene expression and chromatin state of such induced reprogrammed stem cells are similar to those of ES cells. Notably, the cells—derived from mouse fibroblasts—can form viable chimaeras, can contribute to the germ line and can generate live late-term embryos when injected into tetraploid blastocysts. Our results show that the biological potency and epigenetic state of in-vitro-reprogrammed induced pluripotent stem cells are indistinguishable from those of ES cells. IS - 7151 JF - Nature SN - 0028-0836 EP - 324 TI - In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state PB - Nature Publishing Group VL - 448 SP - 318 KW - nsf AU - Wernig, Marius AU - Meissner, Alexander AU - Foreman, Ruth AU - Brambrink, Tobias AU - Ku, Manching AU - Hochedlinger, Konrad AU - Bernstein, Bradley AU - Jaenisch, Rudolf PY - 2007/06/06/ UR - http://dx.doi.org/10.1038/nature05944 ER -