Motile detergent-extracted cells of Tetrahymena and Chlamydomonas.

@article{citeulike:3544910, abstract = {Tetrahymena and Chlamydomonas cells treated with high (0.25-0.5\%) concentrations of the detergent Nonidet P-40 in appropriate buffers retain the shape of the intact cells but are devoid of any ciliary activity unless supplied with MgATP. ATP causes them to swim actively, with beat parameters and swimming patterns indistinguishable from those of intact cells. Both types of detergent-extracted cells are completely devoid of ciliary membranes. The Tetrahymena preparations also lack all cellular membranes, whereas cellular membranes remain intact in the Chlamydomonas preparations. Experiments demonstrating the effects of ATP, ADP, vanadate, erythro-9-[3-2-(hydroxynonyl)]-adenine, and Ca++ are described to illustrate the use of these detergent-extracted cells in research on ciliary motility.}, author = {Goodenough, U. W.}, citeulike-article-id = {3544910}, issn = {0021-9525}, journal = {The Journal of cell biology}, keywords = {lc4, thesis}, month = {June}, number = {6}, pages = {1610--1621}, posted-at = {2008-11-16 21:53:40}, priority = {2}, title = {Motile detergent-extracted cells of Tetrahymena and Chlamydomonas.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/6602134}, volume = {96}, year = {1983} }

TY - JOUR ID - citeulike:3544910 L3 - citeulike-article-id:3544910 N2 - Tetrahymena and Chlamydomonas cells treated with high (0.25-0.5%) concentrations of the detergent Nonidet P-40 in appropriate buffers retain the shape of the intact cells but are devoid of any ciliary activity unless supplied with MgATP. ATP causes them to swim actively, with beat parameters and swimming patterns indistinguishable from those of intact cells. Both types of detergent-extracted cells are completely devoid of ciliary membranes. The Tetrahymena preparations also lack all cellular membranes, whereas cellular membranes remain intact in the Chlamydomonas preparations. Experiments demonstrating the effects of ATP, ADP, vanadate, erythro-9-[3-2-(hydroxynonyl)]-adenine, and Ca++ are described to illustrate the use of these detergent-extracted cells in research on ciliary motility. IS - 6 JF - The Journal of cell biology SN - 0021-9525 EP - 1621 TI - Motile detergent-extracted cells of Tetrahymena and Chlamydomonas. VL - 96 SP - 1610 KW - lc4 KW - thesis AU - Goodenough, UW PY - 1983/06// UR - http://view.ncbi.nlm.nih.gov/pubmed/6602134 ER -