Promoter DNA methylation of oncostatin m receptor-beta as a novel diagnostic and therapeutic marker in colon cancer. Export

@article{citeulike:5727087, abstract = {In addition to genetic changes, the occurrence of epigenetic alterations is associated with accumulation of both genetic and epigenetic events that promote the development and progression of human cancer. Previously, we reported a set of candidate genes that comprise part of the emerging "cancer methylome". In the present study, we first tested 23 candidate genes for promoter methylation in a small number of primary colon tumor tissues and controls. Based on these results, we then examined the methylation frequency of Oncostatin M receptor-beta (OSMR) in a larger number of tissue and stool DNA samples collected from colon cancer patients and controls. We found that OSMR was frequently methylated in primary colon cancer tissues (80\%, 80/100), but not in normal tissues (4\%, 4/100). Methylation of OSMR was also detected in stool DNA from colorectal cancer patients (38\%, 26/69) (cut-off in TaqMan-MSP, 4). Detection of other methylated markers in stool DNA improved sensitivity with little effect on specificity. Promoter methylation mediated silencing of OSMR in cell lines, and CRC cells with low OSMR expression were resistant to growth inhibition by Oncostatin M. Our data provide a biologic rationale for silencing of OSMR in colon cancer progression and highlight a new therapeutic target in this disease. Moreover, detection and quantification of OSMR promoter methylation in fecal DNA is a highly specific diagnostic biomarker for CRC.}, author = {Kim, Myoung Sook S. and Louwagie, Joost and Carvalho, Beatriz and Terhaar Sive Droste, Jochim S. and Park, Hannah Lui L. and Chae, Young Kwang K. and Yamashita, Keishi and Liu, Junwei and Ostrow, Kimberly Laskie L. and Ling, Shizhang and Guerrero-Preston, Rafael and Demokan, Semra and Yalniz, Zubeyde and Dalay, Nejat and Meijer, Gerrit A. and Van Criekinge, Wim and Sidransky, David}, citeulike-article-id = {5727087}, citeulike-linkout-0 = {http://dx.doi.org/10.1371/journal.pone.0006555}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19662090}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19662090}, doi = {10.1371/journal.pone.0006555}, issn = {1932-6203}, journal = {PloS one}, keywords = {hpv}, number = {8}, posted-at = {2009-09-06 04:48:41}, priority = {2}, title = {Promoter DNA methylation of oncostatin m receptor-beta as a novel diagnostic and therapeutic marker in colon cancer.}, url = {http://dx.doi.org/10.1371/journal.pone.0006555}, volume = {4}, year = {2009} }

TY - JOUR ID - citeulike:5727087 L3 - citeulike-article-id:5727087 N2 - In addition to genetic changes, the occurrence of epigenetic alterations is associated with accumulation of both genetic and epigenetic events that promote the development and progression of human cancer. Previously, we reported a set of candidate genes that comprise part of the emerging "cancer methylome". In the present study, we first tested 23 candidate genes for promoter methylation in a small number of primary colon tumor tissues and controls. Based on these results, we then examined the methylation frequency of Oncostatin M receptor-beta (OSMR) in a larger number of tissue and stool DNA samples collected from colon cancer patients and controls. We found that OSMR was frequently methylated in primary colon cancer tissues (80%, 80/100), but not in normal tissues (4%, 4/100). Methylation of OSMR was also detected in stool DNA from colorectal cancer patients (38%, 26/69) (cut-off in TaqMan-MSP, 4). Detection of other methylated markers in stool DNA improved sensitivity with little effect on specificity. Promoter methylation mediated silencing of OSMR in cell lines, and CRC cells with low OSMR expression were resistant to growth inhibition by Oncostatin M. Our data provide a biologic rationale for silencing of OSMR in colon cancer progression and highlight a new therapeutic target in this disease. Moreover, detection and quantification of OSMR promoter methylation in fecal DNA is a highly specific diagnostic biomarker for CRC. IS - 8 JF - PloS one SN - 1932-6203 TI - Promoter DNA methylation of oncostatin m receptor-beta as a novel diagnostic and therapeutic marker in colon cancer. VL - 4 KW - hpv AU - Kim, Myoung Sook AU - Louwagie, Joost AU - Carvalho, Beatriz AU - Terhaar Sive Droste, Jochim AU - Park, Hannah Lui AU - Chae, Young Kwang AU - Yamashita, Keishi AU - Liu, Junwei AU - Ostrow, Kimberly Laskie AU - Ling, Shizhang AU - Guerrero-Preston, Rafael AU - Demokan, Semra AU - Yalniz, Zubeyde AU - Dalay, Nejat AU - Meijer, Gerrit AU - Van Criekinge, Wim AU - Sidransky, David PY - 2009/// UR - http://dx.doi.org/10.1371/journal.pone.0006555 ER -