Characterization of physical interactions of the putative transcriptional adaptor, ADA2, with acidic activation domains and TATA-binding protein. Export

@article{citeulike:1954100, abstract = {RNA polymerase II transcription requires functional interactions between activator proteins bound to upstream DNA sites and general factors bound to the core promoter. Accessory transcription factors, such as adaptors and coactivators, have important, but still unclear, roles in the activation process. We tested physical interactions of the putative adaptor ADA2 with activation domains derived from acidic activator proteins and with certain general transcription factors. ADA2 associated with the herpesvirus VP16 and yeast GCN4 activation domains but not with the activation domain of yeast HAP4, which previously was shown to be independent of ADA2 function in vivo and in vitro. Furthermore, the amino terminus of ADA2 directly interacted with the VP16 activation domain, suggesting that ADA2 provides determinants for interaction between activation domains and the adaptor complex. Both TATA-binding protein (TBP) and TFIIB have previously been shown to interact directly with the VP16 activation domain in vitro (Stringer, K. F., Ingles, C. J., and Greenblatt, J. (1990) Nature 345, 783-786; Lin, Y. S., Ha, I., Maldonado, E., Reinberg, D., and Green, M. R. (1991) Nature 353, 569-571). Interestingly, when binding was tested between VP16 and these general factors in yeast nuclear extracts, both factors interacted with VP16, but only the TBP/VP16 association was dependent on ADA2. In addition, ADA2 physically associated with TBP, but not with TFIIB. These results suggest that the role of ADA2 in transcriptional activation is to promote physical interaction between activation domains and TBP.}, address = {Wistar Institute, Philadelphia, Pennsylvania 19104, USA.}, author = {Barlev, N. A. and Candau, R. and Wang, L. and Darpino, P. and Silverman, N. and Berger, S. L.}, citeulike-article-id = {1954100}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/7642611}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=7642611}, day = {18}, issn = {0021-9258}, journal = {J Biol Chem}, keywords = {ada2, bzip}, month = {August}, number = {33}, pages = {19337--19344}, posted-at = {2007-11-21 20:22:57}, priority = {2}, title = {Characterization of physical interactions of the putative transcriptional adaptor, ADA2, with acidic activation domains and TATA-binding protein.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/7642611}, volume = {270}, year = {1995} }

TY - JOUR ID - citeulike:1954100 L3 - citeulike-article-id:1954100 N2 - RNA polymerase II transcription requires functional interactions between activator proteins bound to upstream DNA sites and general factors bound to the core promoter. Accessory transcription factors, such as adaptors and coactivators, have important, but still unclear, roles in the activation process. We tested physical interactions of the putative adaptor ADA2 with activation domains derived from acidic activator proteins and with certain general transcription factors. ADA2 associated with the herpesvirus VP16 and yeast GCN4 activation domains but not with the activation domain of yeast HAP4, which previously was shown to be independent of ADA2 function in vivo and in vitro. Furthermore, the amino terminus of ADA2 directly interacted with the VP16 activation domain, suggesting that ADA2 provides determinants for interaction between activation domains and the adaptor complex. Both TATA-binding protein (TBP) and TFIIB have previously been shown to interact directly with the VP16 activation domain in vitro (Stringer, K. F., Ingles, C. J., and Greenblatt, J. (1990) Nature 345, 783-786; Lin, Y. S., Ha, I., Maldonado, E., Reinberg, D., and Green, M. R. (1991) Nature 353, 569-571). Interestingly, when binding was tested between VP16 and these general factors in yeast nuclear extracts, both factors interacted with VP16, but only the TBP/VP16 association was dependent on ADA2. In addition, ADA2 physically associated with TBP, but not with TFIIB. These results suggest that the role of ADA2 in transcriptional activation is to promote physical interaction between activation domains and TBP. IS - 33 JF - J Biol Chem SN - 0021-9258 AD - Wistar Institute, Philadelphia, Pennsylvania 19104, USA. EP - 19344 TI - Characterization of physical interactions of the putative transcriptional adaptor, ADA2, with acidic activation domains and TATA-binding protein. VL - 270 SP - 19337 KW - ada2 KW - bzip AU - Barlev, NA AU - Candau, R AU - Wang, L AU - Darpino, P AU - Silverman, N AU - Berger, SL PY - 1995/08/18/ UR - http://view.ncbi.nlm.nih.gov/pubmed/7642611 ER -