Transcriptional Activation of Metalloid Tolerance Genes in Saccharomyces cerevisiae Requires the AP-1-like Proteins Yap1p and Yap8p Export

@article{citeulike:2086444, abstract = {All organisms are equipped with systems for detoxification of the metalloids arsenic and antimony. Here, we show that two parallel pathways involving the AP-1-like proteins Yap1p and Yap8p are required for acquisition of metalloid tolerance in the budding yeast S. cerevisiae. Yap8p is demonstrated to reside in the nucleus where it mediates enhanced expression of the arsenic detoxification genes ACR2 and ACR3. Using chromatin immunoprecipitation assays, we show that Yap8p is associated with the ACR3 promoter in untreated as well as arsenic-exposed cells. Like for Yap1p, specific cysteine residues are critical for Yap8p function. We further show that metalloid exposure triggers nuclear accumulation of Yap1p and stimulates expression of antioxidant genes. Yap1p mutants that are unable to accumulate in the nucleus during H2O2 treatment showed nearly normal nuclear retention in response to metalloid exposure. Thus, our data are the first to demonstrate that Yap1p is being regulated by metalloid stress and to indicate that this activation of Yap1p operates in a manner distinct from stress caused by chemical oxidants. We conclude that Yap1p and Yap8p mediate tolerance by controlling separate subsets of detoxification genes and propose that the two AP-1-like proteins respond to metalloids through distinct mechanisms. 10.1091/mbc.E03-04-0236}, author = {Wysocki, Robert and Fortier, Pierre-Karl and Maciaszczyk, Ewa and Thorsen, Michael and Leduc, Anick and Odhagen, Asa and Owsianik, Grzegorz and Ulaszewski, Stanislaw and Ramotar, Dindial and Tamas, Markus J.}, citeulike-article-id = {2086444}, citeulike-linkout-0 = {http://dx.doi.org/10.1091/mbc.E03-04-0236}, citeulike-linkout-1 = {http://www.molbiolcell.org/cgi/content/abstract/15/5/2049}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/14978214}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=14978214}, day = {1}, doi = {10.1091/mbc.E03-04-0236}, journal = {Mol. Biol. Cell}, keywords = {acr1, arr1, bzip, ypr199c}, month = {May}, number = {5}, pages = {2049--2060}, posted-at = {2007-12-10 18:30:21}, priority = {2}, title = {Transcriptional Activation of Metalloid Tolerance Genes in Saccharomyces cerevisiae Requires the AP-1-like Proteins Yap1p and Yap8p}, url = {http://dx.doi.org/10.1091/mbc.E03-04-0236}, volume = {15}, year = {2004} }

TY - JOUR ID - citeulike:2086444 L3 - citeulike-article-id:2086444 N2 - All organisms are equipped with systems for detoxification of the metalloids arsenic and antimony. Here, we show that two parallel pathways involving the AP-1-like proteins Yap1p and Yap8p are required for acquisition of metalloid tolerance in the budding yeast S. cerevisiae. Yap8p is demonstrated to reside in the nucleus where it mediates enhanced expression of the arsenic detoxification genes ACR2 and ACR3. Using chromatin immunoprecipitation assays, we show that Yap8p is associated with the ACR3 promoter in untreated as well as arsenic-exposed cells. Like for Yap1p, specific cysteine residues are critical for Yap8p function. We further show that metalloid exposure triggers nuclear accumulation of Yap1p and stimulates expression of antioxidant genes. Yap1p mutants that are unable to accumulate in the nucleus during H2O2 treatment showed nearly normal nuclear retention in response to metalloid exposure. Thus, our data are the first to demonstrate that Yap1p is being regulated by metalloid stress and to indicate that this activation of Yap1p operates in a manner distinct from stress caused by chemical oxidants. We conclude that Yap1p and Yap8p mediate tolerance by controlling separate subsets of detoxification genes and propose that the two AP-1-like proteins respond to metalloids through distinct mechanisms. 10.1091/mbc.E03-04-0236 IS - 5 JF - Mol. Biol. Cell EP - 2060 TI - Transcriptional Activation of Metalloid Tolerance Genes in Saccharomyces cerevisiae Requires the AP-1-like Proteins Yap1p and Yap8p VL - 15 SP - 2049 KW - acr1 KW - arr1 KW - bzip KW - ypr199c AU - Wysocki, Robert AU - Fortier, Pierre-Karl AU - Maciaszczyk, Ewa AU - Thorsen, Michael AU - Leduc, Anick AU - Odhagen, Asa AU - Owsianik, Grzegorz AU - Ulaszewski, Stanislaw AU - Ramotar, Dindial AU - Tamas, Markus PY - 2004/05/01/ UR - http://dx.doi.org/10.1091/mbc.E03-04-0236 ER -