Evidence for regulation of reinitiation in translational control of GCN4 mRNA. Export

@article{citeulike:2148874, abstract = {Translational control of the GCN4 gene of Saccharomyces cerevisiae is mediated by four upstream open reading frames (URFs) present in the leader of GCN4 mRNA. URFs 3 and 4 efficiently repress GCN4 expression in normal growth conditions; URFs 1 and 2 are required to overcome this repression in amino acid-starved cells. lacZ fusions to URFs 3 and 4 were used to determine the translational event that is regulated at these sequences by URFs 1 and 2. URF3-lacZ, URF4-lacZ, and GCN4-lacZ fusions are affected similarly by URFs 1 and 2 when no other URFs are present in the leader: expression from all three fusions is reduced by an amount slightly greater in repressing than in derepressing conditions. These results are inconsistent with models that postulate a differential effect of URFs 1 and 2 on initiation or elongation rates at URFs 3 and 4 versus the GCN4 coding sequences. We propose that the efficiency of reinitiation at the GCN4 AUG codon after translation of URFs 3 and 4 is the translational event that is stimulated in derepressing conditions by URFs 1 and 2.}, address = {Unit of Molecular Genetics of Lower Eukaryotes, National Institute of Child Health and Human Development, Bethesda, MD 20892.}, author = {Hinnebusch, A. G. and Jackson, B. M. and Mueller, P. P.}, citeulike-article-id = {2148874}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/3050993}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=3050993}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, month = {October}, number = {19}, pages = {7279--7283}, posted-at = {2007-12-19 23:02:53}, priority = {2}, title = {Evidence for regulation of reinitiation in translational control of GCN4 mRNA.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/3050993}, volume = {85}, year = {1988} }

TY - JOUR ID - citeulike:2148874 L3 - citeulike-article-id:2148874 N2 - Translational control of the GCN4 gene of Saccharomyces cerevisiae is mediated by four upstream open reading frames (URFs) present in the leader of GCN4 mRNA. URFs 3 and 4 efficiently repress GCN4 expression in normal growth conditions; URFs 1 and 2 are required to overcome this repression in amino acid-starved cells. lacZ fusions to URFs 3 and 4 were used to determine the translational event that is regulated at these sequences by URFs 1 and 2. URF3-lacZ, URF4-lacZ, and GCN4-lacZ fusions are affected similarly by URFs 1 and 2 when no other URFs are present in the leader: expression from all three fusions is reduced by an amount slightly greater in repressing than in derepressing conditions. These results are inconsistent with models that postulate a differential effect of URFs 1 and 2 on initiation or elongation rates at URFs 3 and 4 versus the GCN4 coding sequences. We propose that the efficiency of reinitiation at the GCN4 AUG codon after translation of URFs 3 and 4 is the translational event that is stimulated in derepressing conditions by URFs 1 and 2. IS - 19 JF - Proc Natl Acad Sci U S A SN - 0027-8424 AD - Unit of Molecular Genetics of Lower Eukaryotes, National Institute of Child Health and Human Development, Bethesda, MD 20892. EP - 7283 TI - Evidence for regulation of reinitiation in translational control of GCN4 mRNA. VL - 85 SP - 7279 AU - Hinnebusch, AG AU - Jackson, BM AU - Mueller, PP PY - 1988/10// UR - http://view.ncbi.nlm.nih.gov/pubmed/3050993 ER -